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目的探讨高强度聚焦超声(HIFU)协同紫杉醇热敏脂质体(PTL)对小鼠Lewis肺癌的治疗效果。方法建立Lewis肺癌小鼠模型,按肿瘤大小区组随机分为6组,对照组(Con)、单纯HIFU组(HI-FU)、紫杉醇普通注射液组(PTX)、紫杉醇普通注射液联合HIFU组(PTX+HIFU)、紫杉醇热敏脂质体组(PTL)、紫杉醇热敏脂质体联合HIFU组(PTL+HIFU)。治疗后,观察肿瘤体积和重量变化,计算抑瘤率,并对肿瘤进行HE染色,观察肿瘤组织病理形态学变化。结果 PTL+HIFU组的抑瘤率为71.6%,显著高于PTX+HIFU组的抑瘤率50.0%(P<0.05),且前两组均高于PTX组(21.3%,P<0.05)和PTL组(23.4%,P<0.05),PTX组和PTL组的抑瘤率未见明显差异(P>0.05)。同时,HIFU联合两种不同剂型紫杉醇治疗组较单纯紫杉醇药物治疗组,肿瘤细胞坏死范围和程度均较显著,以HIFU联合紫杉醇热敏脂质体组最明显。单纯紫杉醇药物治疗的两组,肿瘤坏死情况差异不明显。结论高强度聚焦超声协同紫杉醇热敏脂质体治疗小鼠Lewis肺癌具有明显的抑瘤效果。HIFU可以作为热敏脂质体靶向治疗肿瘤的热源。
Objective To investigate the therapeutic effect of high intensity focused ultrasound (HIFU) combined with paclitaxel thermosensitive liposome (PTL) on Lewis lung carcinoma in mice. Methods Lewis lung cancer mouse model was established and randomly divided into 6 groups: control group (Con), simple HIFU group (HI-FU), paclitaxel normal injection group (PTX) and paclitaxel normal injection combined with HIFU group (PTX + HIFU), paclitaxel thermosensitive liposomes (PTL), paclitaxel thermosensitive liposomes combined with HIFU group (PTL + HIFU). After treatment, the tumor volume and weight change were observed, the tumor inhibition rate was calculated, and the tumor was stained with HE to observe the histopathological changes of the tumor tissue. Results The tumor inhibition rate in PTL + HIFU group was 71.6%, which was significantly higher than that in PTX + HIFU group (50.0%, P <0.05), and both groups were significantly higher than those in PTX group (21.3%, P <0.05) PTL group (23.4%, P <0.05). There was no significant difference in tumor inhibition rate between PTX group and PTL group (P> 0.05). At the same time, HIFU combined with two different forms of paclitaxel treatment group than the paclitaxel drug treatment group, the extent and extent of tumor necrosis were significant, with HIFU combined with paclitaxel thermosensitive liposomes the most obvious. Simple paclitaxel drug treatment of the two groups, the difference was not obvious tumor necrosis. Conclusion High-intensity focused ultrasound combined with paclitaxel thermosensitive liposomes has obvious anti-tumor effect on Lewis lung cancer in mice. HIFU can be used as a heat-sensitive liposome targeted treatment of cancer heat source.