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为明确中国福建省番茄灰霉病菌对氟啶胺的敏感性及其与不同杀菌剂的交互抗性,采用菌丝生长速率法测定了106株采自福建省主要番茄产区的番茄灰霉病菌对氟啶胺的敏感性。结果表明,氟啶胺对福建省番茄灰霉病菌菌丝生长的EC50值在0.003 7~0.045 2μg/m L之间,平均值为(0.022 1±0.009 8)μg/m L,其敏感性频率分布呈连续单峰曲线,符合正态分布,因此可将该EC50平均值(0.022 1±0.009 8)μg/m L作为福建省番茄灰霉病菌对氟啶胺的敏感基线,用于其田间抗药性监测。从106株菌株中选择15株对氟啶胺敏感性不同的菌株,测定了其对嘧霉胺、异菌脲、腐霉利和啶氧菌酯的敏感性。结果表明,供试5种杀菌剂对15株番茄灰霉病菌菌丝生长的平均抑制活性依次为氟啶胺>异菌脲>腐霉利>啶氧菌酯>嘧霉胺,氟啶胺与异菌脲、腐霉利、啶氧菌酯和嘧霉胺之间均不存在交互抗性。
In order to clarify the sensitivity of Botrytis cinerea to fluazinam in Fujian Province of China and its interaction with different fungicides, the mycelia growth rate method was used to determine the susceptibility of Botrytis cinerea to tomato in 106 major tomato producing areas in Fujian Province Sensitivity to fluazinam. The results showed that the EC50 values of fluazinam on the mycelial growth of Botrytis cinerea in Fujian province ranged from 0.003 7 to 0.045 2 μg / m L with an average of (0.022 1 ± 0.009 8) μg / m L. The sensitivity frequency The results showed that the average EC50 (0.022 1 ± 0.009 8) μg / m L was the sensitive baseline for fosfomycin in Fujian Province for its field resistance Drug monitoring. Fifteen strains were selected from 106 strains, which were sensitive to fluazinam and their susceptibility to pyrimethanil, iprodione, procymidone and picoxystrobin was determined. The results showed that the average inhibitory activities of five fungicides on the mycelial growth of 15 tomato gray mold fungi were as follows: fluazinam> iprodione> picoxystrobin> pyrimethanil, There was no cross-resistance between iprodione, procymidone, picoxystrobin and pyrimethanil.