目的　运用噬菌体抗体库技术 ,制备人源性抗AFPFab段 ,为进一步的研究和临床运用打下基础。方法　用RT PCR方法扩增人免疫球蛋白重链Fd及κ链基因 ,构建噬菌体抗体库 ,筛选出人抗AFPFab片断 ,用ELISA检测其特异性。结果　初步克隆出人抗AFPFab ,该Fab具有结合AFP的活性。结论　噬菌体抗体库技术绕过了细胞杂交这一步骤 ,较好的解决了人体杂交瘤低效的难题 ,为人源性单抗的制备开辟了广阔的前景。 Objective To prepare human anti-AFPFab fragment by phage antibody library technology and lay the foundation for further research and clinical application. Methods The human immunoglobulin heavy chain Fd and κ chain genes were amplified by RT-PCR. The phage antibody library was constructed. The human anti-AFPFab fragment was screened and its specificity was detected by ELISA. As a result, a human anti-AFP Fab was initially cloned, which had the activity of binding AFP. Conclusion The phage antibody library technology bypasses the step of cell hybridization and solves the problem of inefficient human hybridoma. It opens up a broad prospect for the preparation of human monoclonal antibodies.