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以前研究发现,辽宁地区大豆生长期间及收获期土壤中胞囊孵出的二龄幼虫量很少,推测线虫卵的休眠与大豆生长时期或季节相关。为明确该地区大豆胞囊线虫的休眠特点,2002-2003年采用田间随机多点取样、室内分离及模拟自然条件孵化等方法对大豆胞囊线虫的休眠进行深入研究。结果表明:在生长季节,感病品种辽豆10根围土壤中的白色雌虫、卵囊及褐色的胞囊均可孵出二龄幼虫,且孵化持续时间较长,第21d仍有幼虫孵出,白色雌虫及卵囊内的卵孵化率高于褐色胞囊;不同作物对其根围土壤中胞囊内卵的孵化影响不大,寄主作物大豆、非寄主作物玉米根围及休闲地土壤中的胞囊在条件适宜均可孵出二龄幼虫;季节对胞囊内卵的孵化有较大的影响,出苗期孵化率最高,收获期最低,2周时平均1个胞囊孵出幼虫分别为83.8和9.7条;胞囊皮对线虫卵的孵化有显著的影响。表明沈阳地区大豆胞囊线虫在正常和逆境条件下均有部分卵表现休眠。
Previous studies found that the amount of second-instar larvae hatched in the cytoplasm of soils during the soybean growing and harvesting periods in Liaoning was very small, suggesting that the dormancy of the nematode eggs is related to the season or season of soybean growth. In order to clarify the dormancy characteristics of Soybean cyst nematode in this area, the dormancy of Soybean cyst nematode was studied in 2002-2003 by random sampling in multiple fields, indoor separation and incubation in simulated natural conditions. The results showed that in the growing season, the white females, oocysts and brown cysts of 10 susceptible cultivars in Liaozu were all hatched second instar larvae with longer larvae hatching and larvae hatching on the 21th day The hatching rate of egg in white female and oocyst was higher than that in brown cyst. Different crops had little effect on hatching of cysts in the cytoplasm of rhizosphere soil. The host crop soybean, non-host crop corn rhizosphere and fallow In the soil, the cysts could hatch second-instar larvae under suitable conditions. The seasons had a significant influence on the hatching of cysts in the cysts, with the highest hatching rate at hatching stage and the lowest harvesting period. One cyst hatched at 2 weeks The larvae were 83.8 and 9.7, respectively. The cyst envelopes had a significant effect on the incubation of eggs. Soybean cyst nematode in Shenyang showed that some eggs showed dormancy under both normal and adversity conditions.