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目的建立经腹腔注射途经给予造模致敏原的BN大鼠致敏动物模型。方法分别在实验第1、5和10天,经腹腔注射给予不同性别(雌性和雄性)和不同周龄(4周龄和8周龄)的BN大鼠,不同剂量(1.00、0.10和0.01mg)的造模致敏原———卵清蛋白(OVA),观察35天。分别于第28、35天内眦取血分离血清,用ELISA方法检测血清中OVA特异性IgE。结果与阴性对照组相比,雌性8周龄BN大鼠中、高剂量组第28、35天血清中OVA特异性IgE浓度显著升高(P<0.05);雄性8周龄BN大鼠低、中剂量组血清中OVA特异性IgE浓度在第28天显著升高(P<0.05),高剂量组血清中OVA特异性IgE浓度在第35天显著升高(P<0.05);雄性4周龄BN大鼠,高剂量组血清中OVA特异性IgE浓度在第35天显著升高(P<0.05)。结论经腹腔注射途径给予不同性别和周龄的BN大鼠不同剂量的OVA,雌性大鼠比雄性大鼠更敏感;8周龄大鼠比4周龄大鼠更敏感;0.10mg或1.00mg的致敏剂量较适合。因此,选用雌性8周龄BN大鼠,经腹腔注射给予0.10mg或1.00mgOVA,35天后即可建立比较理想的BN大鼠致敏动物模型。
OBJECTIVE: To establish a sensitized animal model of BN rats sensitized with propolis via intraperitoneal injection. Methods BN rats of different sexes (female and male) and different weeks (4 weeks and 8 weeks old) were administrated by intraperitoneal injection on the 1st, 5th and 10th day of experiment. The rats in different doses (1.00, 0.10 and 0.01mg ) Modeling allergen --- ovalbumin (OVA), observed for 35 days. On the 28th and 35th day respectively, blood samples were collected for serum isolation, and serum specific OVA was detected by ELISA. Results Compared with the negative control group, the serum concentrations of OVA-specific IgE in the high-dose and high-dose groups of 8-week-old BN rats were significantly increased at 28 and 35 days (P <0.05) The concentration of OVA-specific IgE in the middle-dose group increased significantly on the 28th day (P <0.05), and the serum concentration of OVA-specific IgE in the high dose group increased significantly on the 35th day (P <0.05) In BN rats, the concentration of serum OVA-specific IgE in high-dose group increased significantly on the 35th day (P <0.05). CONCLUSIONS: Different doses of OVA were given to BN rats of different sexes and weeks of age by intraperitoneal injection. Female rats were more sensitive than male rats; 8-week-old rats were more sensitive than 4-week-old rats; 0.10mg or 1.00mg Sensitization dose is more suitable. Therefore, female 8-week-old BN rats were selected and given intraperitoneal injection of 0.10mg or 1.00mgOVA, 35 days later to establish an ideal animal model of BN rat sensitization.