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目的:探讨骨髓间充质干细胞(MSCs)移植对脑缺血大鼠神经功能恢复及突触可塑性的影响。方法:采用大鼠大脑中动脉缺血模型,分为假手术组、模型组、PBS组和MSCs组,研究脑缺血24h后移植MSCs的大鼠神经功能缺损评分(NSS);分别测定梗死灶周围脑组织突触素(SYN)和脑源性神经营养因子(BDNF)mRNA的表达;电镜及免疫电镜下观察突触结构的变化。结果:与模型组及PBS组大鼠相比,MSCs组的NSS评分较低,SYN及BDNF mRNA的表达则明显较高;电镜检查示MSCs组大鼠突触界面曲率较大,突触后致密物质的厚度增加,突触间隙宽度变窄,突触活性带长度增加;免疫电镜示BrdU阳性细胞和宿主脑神经元形成非成熟的突触样结构。结论:MSCs移植可能通过神经营养效应调节脑缺血周围神经细胞的可塑性改善脑缺血大鼠的神经功能。
Objective: To investigate the effects of bone marrow mesenchymal stem cells (MSCs) transplantation on neural function recovery and synaptic plasticity in cerebral ischemia rats. Methods: Rat models of middle cerebral artery occlusion (MCAO) were divided into four groups: sham operation group, model group, PBS group and MSCs group. Neurological deficit score (NSS) The expression of synaptophysin (SYN) and brain-derived neurotrophic factor (BDNF) mRNA in the surrounding brain tissue was observed. The synaptic structure was observed under electron microscope and electron microscopy. Results: Compared with the model group and PBS group, the NSS score of MSCs group was lower and the expression of SYN and BDNF mRNA were significantly higher. Electron microscopy showed that the MSCs group had larger curvature and more postsynaptic density The thickness of synaptic cleft increased with the thickness of the synaptic cleft, and the length of the synaptic active band increased. Immunoblotting showed that BrdU positive cells and host brain neurons formed immature synaptic structures. CONCLUSION: The transplantation of MSCs may improve neurological function of cerebral ischemic rats by regulating the plasticity of peripheral nerve cells in cerebral ischemia by neurotrophic effect.