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本文利用Ｔｅｔｏｎ基因表达系统建立了一株可诱导ＥＢ病毒ＬＭＰ１基因表达的鼻咽癌细胞株。首先将Ｔｅｔｏｎ基因调控系统的调节质粒ｐＴｅｔｏｎ导入鼻咽癌细胞系ＨＮＥ２中，用ｒｔＴＡ反应性荧光素酶报道基因ｐＴＲＥｌｕｃ挑选高诱导、低背景的克隆，第二轮转染将构建的反应质粒ｐＴＲＥＬＭＰ１导入得到稳定转染的阳性克隆，对各克隆用Ｗｅｓｔｅｒｎ印迹方法进行强力霉素（Ｄｏｘ）诱导效应检测，其中Ｌ７对Ｄｏｘ具有较好的剂量反应依赖性，诱导表达量可增加１０倍左右。ＬＭＰ１在鼻咽癌细胞可调表达对于分析其不同程度的表达在鼻咽癌发生中的作用具有重要价值。
In this paper, Teton gene expression system was used to establish a nasopharyngeal carcinoma cell line that induced EBV LMP1 gene expression. Firstly, the regulatory plasmid pTeton of Teton gene regulation system was introduced into the nasopharyngeal carcinoma cell line HNE2. The rtTA luciferase reporter gene pTREluc was used to select highly induced and low background clones. The second round of transfection The constructed recombinant plasmid pTRE-LMP1 was transfected into a stable transfected positive clone. The Dox induction effect of each clone was detected by Western blotting. L7 had a good dose-response dependence on Dox, and the induced expression level Can increase about 10 times. The expression of LMP1 in nasopharyngeal carcinoma cells is of great value for analyzing the role of different levels of expression of LMP1 in the development of NPC.