Engineered cells have opened up a new avenue for scientists and engineers to achieve specialized biological functions.Nanomaterials,such as silicon nanowires and quantum dots,can establish tight interfaces with cells either extra-or intracellularly,and they have already been widely used to control cellular functions.The future exploration of nanomaterials in cellular engineering may reveal numerous opportunities in both fundamental bioelectric studies and clinic applications.In this review,we highlight several nanomaterials-enabled non-genetic approaches to fabricating engineered cells.First,we briefly review the latest progress in engineered or synthetic cells,such as protocells that create cell-like behaviors from nonliving building blocks,and cells made by genetic or chemical modifications.Next,we illustrate the need for non-genetic cellular engineering with semiconductors and present some examples where chemical synthesis yields complex morphology or functions needed for biointerfaces.We then provide discussions in detail about the semiconductor nanostructure-enabled neural,cardiac,and microbial modulations.We also suggest the need to integrate tissue engineering with semiconductor devices to carry out more complex functions.We end this review by providing our perspectives for future development in non-genetic cellular engineering.