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目的应用胶体金免疫层析技术,研制1种ABO血型抗原检测胶体金试剂条。方法对试剂条在研制过程中牵涉到的各项工艺参数进行优化,包括:标记抗体用量与包被NC膜抗体浓度筛选,加样量及反应时间确定等,并对500例常规全血标本进行检测,评估试剂条的临床准确性。结果确定标记抗体浓度为24μg/m L、抗-A包被浓度为1.2 mg/m L、抗-B包被浓度为1.0 mg/m L,加样量为10-20μL、反应时间5-15 min,临床准确率100%。结论试条具有操作简单,携带方便,准确性高,无需仪器辅助等特点,检测样本不局限于人类新鲜全血,还可以是凝块血、血渍,将来甚至可以检测唾液、尿液的ABO血型,适合流动采血点、法医学现场检验和医院检验科室的血型初筛。
Objective To develop an ABO blood group antigen colloidal gold reagent strip by using colloidal gold immunochromatography. Methods The process parameters involved in the development of the reagent strip were optimized, including the selection of the amount of labeled antibody and the concentration of antibody coated with NC membrane, the determination of the amount of sample and the reaction time, and the determination of 500 routine whole blood samples Test, assess the clinical accuracy of the reagent strip. As a result, it was confirmed that the labeled antibody concentration was 24 μg / mL, the anti-A coating concentration was 1.2 mg / mL, the anti-B coating concentration was 1.0 mg / mL, the sample volume was 10-20 μL and the reaction time was 5-15 min, the clinical accuracy rate of 100%. Conclusion The test strip has the advantages of simple operation, convenient portability, high accuracy and no need of instrument assisting. The test sample is not limited to human fresh whole blood, but also can be clot blood and blood stain. In the future, even the ABO blood type of saliva and urine can be detected , Suitable for mobile blood collection sites, forensic field testing and hospital testing department of blood type screening.