在组织培养过程中使用诱变剂可以提高细胞突变频率,结合采用逆温胁迫,可以筛选耐逆温变异体,而这一途径的实现需要选择合适、有效的诱变剂和高频植株再生技术的建立。青菜及甘蓝外植体离体培养再生植株的能力较低,早期组织培养再生植株方面的报道往往限于通过愈伤组织途径,近年来也有以子叶柄为外植体直接诱导不定芽的报道。本 The use of mutagens in tissue culture can increase the frequency of cell mutation, and in combination with inversion temperature stress, it is possible to screen for temperature-tolerant variants, and the realization of this pathway needs to select appropriate and effective mutagens and high-frequency plant regeneration technology The establishment of. Brassica campestris plants and cabbage explants are less capable of culturing regenerated plants in vitro. Reports of early tissue culture regenerated plants are often limited to callus pathways, and adventitious buds are directly induced by explants of cotyledons in recent years. this