研究了固定化米曲霉菌光学拆分N-乙酰-D,L-丙氨酸反应过程的速率控制步骤。在近似无限浴的条件下,通过测量间歇拆分反应产生的L-丙氨酸的浓度变化规律,判断反应过程中的速率控制步骤。结果表明,底物浓度和反应温度是影响速率控制步骤的重要因素,用得到的有效扩散系数进行模型计算,结果与实验值基本一致。 The rate-controlling steps of the optical resolution of N-acetyl-D, L-alanine in immobilized Aspergillus oryzae were studied. Under the condition of nearly infinite bath, the rate of L-alanine in the reaction was determined by measuring the variation of L-alanine concentration produced by the batchwise resolution reaction. The results show that the substrate concentration and reaction temperature are the important factors that affect the rate control step. The calculated effective diffusivity is used to calculate the model. The results are in good agreement with the experimental values.