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目的 探讨LRP15基因的甲基化在急性白血病(AL)发生、发展过程中的作用及临床意义。方法 采用甲基化特异性PCR(MSP)方法检测COS7、K562、HL60细胞系、9例正常人及73例AL患者骨髓LRP15基因的甲基化状况。结果 (1)细胞系、正常人及白血病标本中均未检测到LRP15基因的缺失。(2)LRP15基因在COS7中无甲基化,在K562、HL60中完全甲基化。(3)LRP15基因在AL(712%)中甲基化阳性率明显高于正常人(0%),差异有统计学意义(P<005)。(4)AL中LRP15甲基化阳性率难治复发组(833%)高于初治组(571%),两者差异有统计学意义(P<005)。结论 LRP15甲基化与白血病发生发展关系密切,可能是抑癌基因。
Objective To investigate the role of methylation of LRP15 gene in the development and progression of acute leukemia (AL) and its clinical significance. Methods Methylation-specific PCR (MSP) was used to detect the methylation status of LRP15 gene in bone marrow of COS7, K562 and HL60 cell lines, 9 normal subjects and 73 AL patients. Results (1) No deletion of LRP15 gene was detected in cell lines, normal subjects and leukemia specimens. (2) LRP15 gene has no methylation in COS7 and complete methylation in K562 and HL60. (3) The positive rate of methylation of LRP15 gene in AL (712%) was significantly higher than that in normal people (0%), the difference was statistically significant (P <005). (4) The positive rate of methylation of LRP15 in AL was higher in refractory relapse group (833%) than in primary treatment group (571%), the difference was statistically significant (P <0.05). Conclusion The methylation of LRP15 is closely related to the occurrence and development of leukemia, which may be tumor suppressor gene.