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目的：探讨Ａｓ２Ｏ３对胃腺癌ＳＧＣ７９０１细胞系的生物学效应及机制。方法：通过ＭＴＴ还原法检测Ａｓ２Ｏ３对该细胞系存活率的影响，从光学显微镜形态观察，流式细胞仪分析，ＤＮＡ凝胶电泳，细胞凋亡原位检测（ＴＵＮＥＬ）进行细胞凋亡的检测。半定量ＲＴＰＣＲ检测基因表达。结果：Ａｓ２Ｏ３处理ＳＧＣ７９０１细胞后，细胞的存活率明显降低，光学显微镜下可见到明显的凋亡细胞，流式细胞仪测定细胞周期的Ｇ１期前有亚２倍体的凋亡峰，ＤＮＡ凝胶电泳显示出典型的凋亡特征：ＤＮＡ有规律断裂形成的梯状图谱，细胞凋亡原位检测发现ＤＮＡ的断裂，并降低细胞ｃｍｙｃ基因的表达。结论：Ａｓ２Ｏ３能诱导人胃腺癌ＳＧＣ７９０１细胞程序化死亡并可能通过降低ｃｍｙｃ基因的表达。
Objective: To investigate the biological effects of As2O3 on gastric adenocarcinoma SGC7901 cell line and its mechanism. Methods: The effect of As2O3 on the cell survival rate was detected by MTT reduction method. The apoptosis was detected by light microscopy, flow cytometry, DNA gel electrophoresis and TUNEL. Semi-quantitative RT-PCR detection of gene expression. Results: As2O3 treated SGC7901 cells, the cell viability was significantly reduced under light microscopy visible apoptotic cells, flow cytometry measured cell cycle before the G1 phase sub-2-day apoptotic peak DNA gel Electrophoresis showed the typical characteristics of apoptosis: DNA ladder formed by regular rupture pattern, DNA apoptosis detected in situ detection of apoptosis, and reduce the expression of cell c myc gene. Conclusion: As2O3 can induce programmed cell death in human gastric adenocarcinoma SGC7901 cells and may reduce the expression of c-myc gene.