论文部分内容阅读
目的探讨血清中可溶性血管内皮生长因子受体1(sFlt-1)的变化及其与子痫前期发病的关系。方法(1)应用半定量RT-PCR技术检测10例重度子痫前期患者(子痫前期组)及10例足月正常妊娠妇女(正常妊娠组)的胎盘组织中sFlt-1mRNA表达水平。(2)应用酶联免疫吸附试验(ELISA)法测定35例重度子痫前期患者(子痫前期1组)及35例正常足月妊娠妇女(正常妊娠1组)外周血中sFlt-1水平。(3)ELISA测定20例重度子痫前期患者(子痫前期2组)及20例正常足月妊娠妇女(正常妊娠2组)胎盘附着处子宫静脉血中sFlt-1水平。(4)ELISA测定10例早孕(早孕组)及10例中孕(中孕组)妇女外周血中sFlt-1水平。结果(1)子痫前期组胎盘组织中sFlt-1mRNA表达水平为0.95±0.04,明显高于正常妊娠组的0.64±0.15,两组比较,差异有统计学意义(P<0.01)。(2)子痫前期1组孕妇外周血清中sFlt-1水平为(5640±3191)ng/L,明显高于正常妊娠1组的(2194±635)ng/L,两组比较,差异有统计学意义(P<0.01)。(3)子痫前期2组孕妇子宫静脉血清中sFlt-1水平为(7673±2296)ng/L,明显高于正常妊娠2组的(3057±785)ng/L,两组比较,差异有统计学意义(P<0.01)。(4)早、中孕组孕妇外周血清中sFlt-1水平分别为(32±20)ng/L及(994±302)ng/L。结论(1)子痫前期患者外周血中sFlt-1水平明显增高;(2)血清中sFlt-1水平随孕周增加而升高,并可能与子痫前期的发病有关。
Objective To investigate the changes of serum soluble vascular endothelial growth factor receptor 1 (sFlt-1) and its relationship with the pathogenesis of preeclampsia. Methods (1) Semi-quantitative RT-PCR was used to detect the expression of sFlt-1mRNA in placenta of 10 patients with severe preeclampsia (preeclampsia group) and 10 normal pregnant women (normal pregnancy group). (2) The level of sFlt-1 in peripheral blood of 35 patients with severe preeclampsia (preeclampsia group 1) and 35 normal pregnant women (normal pregnant group 1) were determined by enzyme linked immunosorbent assay (ELISA). (3) The level of sFlt-1 in uterine venous blood of placenta attached to 20 patients with severe preeclampsia (preeclampsia group 2) and 20 normal pregnant women (normal pregnant group) were detected by ELISA. (4) The levels of sFlt-1 in peripheral blood of 10 pregnant women (early pregnant group) and 10 middle pregnant women (pregnant women) were measured by ELISA. Results (1) The expression level of sFlt-1mRNA in placenta of preeclampsia group was 0.95 ± 0.04, which was significantly higher than that of normal pregnancy group (0.64 ± 0.15). There was significant difference between the two groups (P <0.01). (2) The level of sFlt-1 in pregnant women with preeclampsia (5640 ± 3191 ng / L) was significantly higher than that in normal pregnancy (2194 ± 635) ng / L, the difference was statistically significant Significance (P <0.01). (3) The level of sFlt-1 in uterine vein of pregnant women with preeclampsia was (7673 ± 2296) ng / L, which was significantly higher than that in normal pregnancy (3057 ± 785) ng / L. There was significant difference between the two groups Statistical significance (P <0.01). (4) The levels of sFlt-1 in peripheral blood serum of pregnant women in the early and middle-pregnancy groups were (32 ± 20) ng / L and (994 ± 302) ng / L, respectively. Conclusions (1) The level of sFlt-1 in peripheral blood of preeclampsia patients was significantly higher than that of preeclampsia patients. (2) The level of sFlt-1 in serum increased with gestational weeks increased, which may be related to the pathogenesis of preeclampsia.