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该文应用1.5,2.0,3.0 g/L亚硫酸钠制造新的肺动脉平滑肌细胞化学缺氧模型,并与三气培养箱制造的物理缺氧模型进行对比,采用hematoxylin-eosin staining(HE染色)、Cell Counting Kit-8(CCK-8)来观察亚硫酸钠对细胞的影响,检测细胞体内的活性氧和缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)的基因及蛋白表达量来判断模型的缺氧程度。细胞体内活性氧和HIF-1α的检测结果提示,亚硫酸钠所制造的缺氧模型的缺氧程度随其浓度的升高而升高,虽然2.0 g/L和3.0 g/L亚硫酸钠组对细胞有一定程度的毒性作用,但是1.5 g/L亚硫酸钠组对细胞的损害程度与物理缺氧组相比无明显差异。该研究表明,1.5 g/L亚硫酸钠所制造的肺动脉平滑肌细胞化学缺氧模型较符合肺动脉平滑肌细胞缺氧模型的要求。
In this paper, a new model of pulmonary artery smooth muscle cell hypoxia was made using 1.5, 2.0, 3.0 g / L sodium sulfite and compared with physical hypoxia model made in three-atmosphere incubator. Hematoxylin-eosin staining (HE staining), Cell Counting Kit-8 (CCK-8) was used to observe the effects of sodium sulfite on cells. The levels of reactive oxygen species and hypoxia inducible factor-1α (HIF-1α) Hypoxia level. The results of reactive oxygen species (ROS) and HIF-1α in cells showed that the hypoxia level of hypoxia model made by sodium sulfite increased with the increase of its concentration, although 2.0 g / L and 3.0 g / L sodium sulfite group had certain The degree of toxicity, but 1.5 g / L sodium sulfite damage to cells compared with the physical hypoxia group no significant difference. The study showed that 1.5 g / L sodium sulfite manufactured pulmonary artery smooth muscle cell hypoxia model more in line with pulmonary artery smooth muscle cell hypoxia model requirements.