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以携带３５Ｓ－Ω－Ｂ．ｔ－Ｎｏｓ嵌合基因的双元载体农杆菌ｐＢ４８．２１４和ｐＢ４８２．１５（Ｂ．ｔ基因片断长度分别为２．１ｋｂ和１．８ｋｂ），转化欧美杨叶片和茎段外植体，共获得２２５株转化再生植株。以舞毒蛾（ＬｙｍａｎｔｒｉａｄｉｓｐａｒＬｉｎｎａｅｕｓ）幼虫进行生物测定后，获得杀虫率分别为５５—８０％的抗虫转基因植株共３株（Ｒ－１３５，Ｒ－１２３和Ｒ－１４０），经组培苗和苗圃移栽植株的ＰＣＲ分析，苗圃移栽植株ＰＣＲ产物杂交和Ｓｏｕｔｈｅｒｎｂｌｏｔ分析表明，Ｂ．ｔ毒蛋白基因已整合到上述抗虫植株的细胞ＤＮＡ中，并表达出杀虫活性。
To carry 35S-Ω-B. The binary vector pB48.214 and pB482.15 of t-Nos chimeric gene (the length of B.t gene were 2.1kb and 1.8kb respectively) were transformed into the leaves and stem explants of Populus trichocarpa to obtain 225 Transform regenerated plants. After the larvae of Lymantriadis parnaeinaeus were bioassayed, 3 insect-resistant transgenic plants (R-135, R-123 and R-140) with insecticidal rates of 55-80% PCR analysis of transplanting plants, hybridization of PCR products of nursery transplanting plants and Southern blot analysis indicated that B. The t-protein gene has been integrated into the cellular DNA of the above insect-resistant plants and expresses insecticidal activity.