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利用氟哌酸与铽离子形成配合物以增强铽离子的特征荧光,在pH=5.6的六次甲基四胺溶液介质中,以332umty入射光激发,该体系发射的铽离子的545nm荧强度与氟哌酸的浓度在1.3×10-2~16μg/ml范围内呈线性相关,氟哌酸的绝对检测限可达3.2×10-8μg/ml。用对照品法测定了诺氟沙星胶囊中氟哌酸的含量,结果令人满意。
The use of norfloxacin and terbium ions to form complexes to enhance the characteristic fluorescence of terbium ions, pH = 5.6 hexamethylenetetramine solution medium, with 332umty incident light excitation, the system emits terbium ions 545nm fluorescence The concentration of norfloxacin was linearly correlated with the concentration of norfloxacin in the range of 1.3 × 10-2 ~ 16μg / ml. The absolute limit of detection of norfloxacin was 3.2 × 10-8μg / ml. The content of norfloxacin in norfloxacin capsules was determined by the reference method and the results were satisfactory.