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目的研究中国健康人群趋化性细胞因子配体蛋白CCL3L1(CC chemokine ligand 3-like-1)的拷贝数及其与HIV易感性的关系。方法收集全血标本,提取基因组DNA,应用Realtime PCR法分析其CCL3L1的相对拷贝数。首先选取22例健康人和23例HIV慢性感染者计算其CCL3L1拷贝数,分析我国健康人群CCL3L1平均拷贝数情况,并分析HIV感染和CCL3L1拷贝数的关系;选取男性同性恋早期感染HIV阳性者29例和HIV阴性者57例,分析CCL3L1基因拷贝数与HIV易感性的关系。结果我国人群CCL3L1平均拷贝数约为3;HIV慢性感染者与健康人无显著性差异(P=0.880);同性恋HIV阴性者CCL3L1拷贝数显著高于同性恋HIV阳性者(P=0.000)。结论我国健康人CCL3L1平均拷贝数为3,CCL3L1拷贝数与HIV易感性关系密切。
Objective To study the copy number of CC chemokine ligand 3-like-1 (CCL3L1) and its relationship with HIV susceptibility in healthy Chinese population. Methods The whole blood samples were collected and genomic DNA was extracted. The relative copy number of CCL3L1 was analyzed by Realtime PCR. First of all, 22 healthy individuals and 23 chronic HIV infected persons were selected to calculate the CCL3L1 copy number. The average copy number of CCL3L1 in healthy population of China was analyzed. The relationship between HIV infection and the copy number of CCL3L1 was analyzed. Twenty-nine HIV positive cases of homosexuality And 57 cases of HIV-negative, analysis of CCL3L1 gene copy number and the relationship between HIV susceptibility. Results The average copy number of CCL3L1 was about 3 in our country. There was no significant difference between HIV-infected and healthy controls (P = 0.880). The copy number of CCL3L1 was significantly higher in homosexual HIV-negative patients than in homosexual HIV-positive patients (P = 0.000). Conclusion The average copy number of CCL3L1 in Chinese healthy people is 3, and the copy number of CCL3L1 is closely related to HIV susceptibility.