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目的检测健侧颈7神经根移位术后脊髓基因表达的变化,探讨神经根移位术后脊髓可塑性形成的机制。方法30只雄性C57BC/6小鼠,体重20 g,随机分为实验组与对照组,每组15只。实验组又分为3个亚组:损伤即刻修复3个月组,损伤3个月、修复3个月组,损伤1个月、修复6个月组,每组5只。按照上述分组在制作全臂丛损伤模型后行健侧颈7移位术,于不同时间点取材应用Biostar M-140s基因芯片检测小鼠脊髓组织基因表达的变化,并与对照组进行比较。结果损伤即刻修复3个月组,损伤3个月、修复3个月组,损伤1个月、修复6个月组脊髓组织分别有55,6和118个基因发生表达水平的改变。其中编码免疫球蛋白重链、chitinase 3的基因在损伤即刻修复3个月组和损伤1个月、修复6个月组均表达增加。编码血管紧张素、collagen、myosin的基因在损伤即刻修复3个月组表达减少,而在损伤1个月、修复6个月组表达增加。突触蛋白synaptotagmin和synaptobrevin在损伤3个月、修复3个月组基因表达改变不明显,而在损伤1个月、修复6个月组表达明显升高。结论健侧颈7神经根移位术能引起脊髓组织基因表达水平发生变化,这种变化呈时间依赖性。免疫应答发生于术后各个阶段,而突触可塑性的形成主要发生在术后6个月。
Objective To detect the changes of spinal cord gene expression after contralateral cervical 7 nerve root transposition and to explore the mechanism of spinal cord plasticity after nerve root transposition. Methods Thirty male C57BC / 6 mice weighing 20 g were randomly divided into experimental group and control group, with 15 rats in each group. The experimental group was divided into three subgroups: the injury was repaired immediately for 3 months, the injury was 3 months, the 3 month group was repaired, the injury was 1 month and the 6-month repair group was 5 rats. According to the above-mentioned grouping, the neck-7 shift was performed after making the brachial plexus injury model. The changes of gene expression in the spinal cord of mice were detected by Biostar M-140s gene chip at different time points and compared with the control group. Results Immediate injury repaired the 3-month group, injured 3 months, repaired the 3-month group, injured 1 month, and repaired the 6-month group, and there were 55, 6 and 118 genes in the spinal cord respectively. Among them, the gene encoding immunoglobulin heavy chain, chitinase 3, was rapidly repaired in the 3-month injury group and 1-month injury group, and the expression of chitinase 3 increased in 6-month repair group. The genes encoding angiotensin, collagen and myosin decreased in the immediate repair group at 3 months and increased at 6 months after injury at 1 month. Synaptotagmin and synaptobrevin showed no significant changes in gene expression at 3 months and 3 months after injury, but significantly increased at 6 months after injury at 1 month. Conclusion The transposition of the contralateral neck and 7 nerve roots can cause the changes of gene expression in spinal cord tissue. The changes are time-dependent. Immune response occurs in all stages of postoperative synaptic plasticity occurs mainly in 6 months after surgery.