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目的 :研究 p16基因突变与喉癌生物学行为及预后的关系。方法 :应用 PCR- SSCP对 2 4例喉癌组织及 10例喉部良性病变组织中 p16基因 (exon2 )进行检测。结果 :喉癌突变检出率为 6 2 .5 % (15 / 2 4) ,而且随着组织分化程度、浸润范围和淋巴结转移的不同而不同 ;喉部良性病变无一例突变。结论 :p16基因突变与喉癌的多种生物学行为有明显关系 (P≤ 0 .0 5 ) ;这种关系有助于判断喉癌的恶性程度及预后。 PCR- SSCP检测喉癌突变 ,具有敏感、简便、重复性好 ,快速而经济等优点 ,是一种适用于临床样本筛选的方法。
Objective: To study the relationship between p16 gene mutation and biological behavior and prognosis of laryngeal cancer. Methods: p16 gene (exon2) in 24 cases of laryngeal carcinoma and 10 cases of benign laryngeal lesions were detected by PCR-SSCP. Results: The detection rate of laryngeal cancer was 62.5% (15/2 4), which was different with the degree of tissue differentiation, infiltration range and lymph node metastasis. No mutation was found in benign laryngeal lesions. CONCLUSION: The mutation of p16 gene has a significant relationship with many biological behaviors of laryngeal carcinoma (P≤0.05). This relationship is helpful to judge the malignancy and prognosis of laryngeal carcinoma. PCR-SSCP detection of laryngeal cancer mutations, with sensitivity, simplicity, good reproducibility, fast and economical, is a suitable screening method for clinical samples.