用反相聚合法合成丙烯酰胺-丙烯酸甲酯交联共聚物,将之用二胺类或多乙烯多胺类胺解制得一系列功能基化载体,并用于米曲霉氨基酰化酶的固定化,考察了载体的各种结构因素对固定化酶活力的影响.选出了一种载体(IAR-2)制备出了固定化酶,详细研究各种因素对固定化酶活力的影响并与溶液酶进行了比较以及用于对N-乙酰-DL-蛋氨酸连续拆分的操作稳定性,该固定化酶活力高(>2000U/g),使用周期长. Acrylamide-methyl acrylate cross-linked copolymer was synthesized by reverse-phase polymerization, and then a series of functionalized carriers were obtained by diamines or polyethylenimine amines and used for the immobilization of Aspergillus oryzae aminoacylase , And investigated the effect of various structural factors on the activity of immobilized enzyme.The immobilized enzyme was prepared by selecting a carrier (IAR-2), and the effect of various factors on the activity of immobilized enzyme was investigated in detail. The enzymes were compared and used for the operational stability of continuous resolution of N-acetyl-DL-methionine, which had high viability (> 2000 U / g) over a long period of use.