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目的:对中国HIV感染者NK细胞、CD8+T细胞及胞内穿孔素、颗粒酶-B表达与CD4+CD25+Foxp3+调节性T细胞水平的相关性进行研究,探讨调节性T细胞在HIV感染中的作用机制。方法:选取73名HIV/AIDS患者(长期不进展组、无症状HIV组、AIDS组),应用流式细胞仪胞内染色技术检测NK细胞、CD8+T细胞数量及胞内穿孔素、颗粒酶-B表达水平,分析其与CD4+CD25+Foxp3+调节性T细胞水平的相关性。结果:CD4+CD25+Foxp3+调节性T细胞百分率与NK细胞、CD8+T淋巴细胞数量呈明显负相关(P<0.01),与CD8+T细胞内穿孔素、颗粒酶-B表达百分率呈明显正相关(P<0.05),与NK细胞内穿孔素、颗粒酶-B表达水平绝对值负相关(P<0.01),与CD8+T细胞内颗粒酶-B表达绝对值呈明显负相关(P<0.01),与CD8+T细胞内穿孔素表达绝对值无明显相关性(P>0.05)。CD4+CD25+Foxp3+调节性T细胞绝对值与CD8+T细胞内穿孔素、颗粒酶-B表达百分率呈明显负相关(P<0.05)。结论:中国HIV感染者细胞毒性淋巴细胞数量功能的变化与调节性T细胞显著相关,提示高水平的调节性T细胞可能与细胞毒性淋巴细胞耗竭相关,可能为导致疾病进展的原因之一。
Objective: To investigate the correlation between the expression of NK cells, CD8 + T cells, perforin and granzyme-B in CD4 + CD25 + Foxp3 + regulatory T cells in HIV-infected Chinese and to explore the role of regulatory T cells in HIV infection In the mechanism of action. Methods: Totally 73 HIV / AIDS patients (long-term non-progressing group, asymptomatic HIV group and AIDS group) were selected. The numbers of NK cells, CD8 + T cells and perforin, B expression levels, and analyze its correlation with CD4 + CD25 + Foxp3 + regulatory T cell levels. Results: The percentage of CD4 + CD25 + Foxp3 + regulatory T cells was significantly negatively correlated with the number of NK cells and CD8 + T lymphocytes (P <0.01), while the expression of perforin and granzyme B in CD8 + T cells was significantly positive (P <0.05), but negatively correlated with the absolute value of perforin and granzyme-B expression in NK cells (P <0.01), and negatively correlated with the absolute value of granzyme B expression in CD8 + T cells (P < 0.01). There was no significant correlation between the expression of perforin and the absolute value of CD8 + T cells (P> 0.05). The absolute value of CD4 + CD25 + Foxp3 + regulatory T cells was negatively correlated with the percentage of perforin and granzyme B in CD8 + T cells (P <0.05). CONCLUSION: The changes in the number and function of cytotoxic lymphocytes in HIV-infected persons in China are significantly associated with regulatory T cells, suggesting that high levels of regulatory T cells may be associated with depletion of cytotoxic lymphocytes and may contribute to the progression of the disease.