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目的:观察肿瘤坏死因子-α(TNF-α)及其受体(sTNF-R1和sTNF-R2)在急性毛细支气管炎患者中的表达,探讨其在急性毛细支气管炎发病机制中的作用。方法:41例急性毛细支气管炎患者来自2008年9月～2009年6月台州市中西医结合医院的患者,酶联免疫吸附试验法检测血清TNF-α、sTNF-R1和sTNF-R2的蛋白浓度。结果:急性毛细支气管炎急性期TNF-α、sTNF-R1和sTNF-R2的蛋白浓度〔分别为(2.66±1.37)、(3.59±1.96)、(11.42±4.76)ng/ml〕显著高于对照组〔分别为(1.85±0.83)、(2.14±0.80)、(4.69±1.91)ng/ml〕(均P<0.01);恢复期sTNF-R1和sTNF-R2的蛋白浓度〔分别为(2.47±1.06)、(6.81±2.39)ng/ml〕显著低于急性期(均P<0.01),而TNF-α的蛋白浓度(2.31±0.96)ng/ml,差异无统计学意义(P>0.05)。sTNF-R1与sTNF-R2的表达水平呈显著正相关(n=107,r=0.56,P<0.01),sTNF-R1和sTNF-R2的表达水平与TNF-α之间均无显著相关性(均P>0.05)。结论:急性毛细支气管炎患者血清中TNF-αs、TNF-R1和sTNF-R2的蛋白浓度增加,可能共同参与了急性毛细支气管炎的炎症过程,可作为病情判断的指标之一。
OBJECTIVE: To observe the expression of tumor necrosis factor-α (TNF-α) and its receptors (sTNF-R1 and sTNF-R2) in patients with acute bronchiolitis and to explore their roles in the pathogenesis of acute bronchiolitis. Methods: Thirty-one patients with acute bronchiolitis were recruited from September 2008 to June 2009 in Taizhou City Hospital of Integrated Traditional and Western Medicine. The serum concentrations of TNF-α, sTNF-R1 and sTNF-R2 were detected by enzyme-linked immunosorbent assay . RESULTS: The protein concentrations of TNF-α, sTNF-R1 and sTNF-R2 in acute stage of acute bronchiolitis were (2.66 ± 1.37), (3.59 ± 1.96) and (11.42 ± 4.76) ng / ml, (1.85 ± 0.83, 2.14 ± 0.80, 4.69 ± 1.91 ng / ml, respectively) (all P <0.01). The protein concentrations of sTNF-R1 and sTNF-R2 in recovery phase were (2.47 ± 1.06 and 6.81 ± 2.39 ng / ml, respectively) were significantly lower than those in the acute phase (all P <0.01), while the concentration of TNF-α was 2.31 ± 0.96 ng / ml, with no significant difference . The expression levels of sTNF-R1 and sTNF-R2 were positively correlated (n = 107, r = 0.56, P <0.01). There was no significant correlation between sTNF-R1 and sTNF- All P> 0.05). CONCLUSIONS: The serum concentrations of TNF-αs, TNF-R1 and sTNF-R2 in patients with acute bronchiolitis may be involved in the inflammatory process of acute bronchiolitis, which may be used as one of the indicators of disease judgment.