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目的探讨细胞毒性T淋巴细胞相关抗原4(CTLA4)基因微卫星多态性与浙江省炎症性肠病(IBD)患者的相关性。方法对118例无血缘关系的IBD患者(99例溃疡性结肠炎,19例克罗恩病)以及140例正常对照者,采用特异性等位基因PCR方法,检测CTLA4外显子4的3′非翻译区包含(AT)n重复序列的等位基因。扩增产物用12%非变性聚丙烯酰胺凝胶电泳,硝酸银染色。结果CTLA4微卫星共有20种等位基因。与正常对照组比较,122bp等位基因频率在溃疡性结肠炎患者(P=0.0001/Pc=0.0025,OR=11.393,95%CI:2.574～50.429)和克罗恩病患者(P=0.0003/Pc=0.0050,OR=21.061,95%CI:3.927～112.94)中均显著增高。结论CTLA4基因微卫星多态性与浙江省IBD患者显著相关。
Objective To investigate the correlation between cytotoxic T lymphocyte antigen 4 (CTLA4) gene microsatellite polymorphism and inflammatory bowel disease (IBD) in Zhejiang Province. Methods 118 unrelated IBD patients (99 ulcerative colitis, 19 Crohn ’s disease) and 140 normal control subjects were enrolled in this study. Specific allele PCR was used to detect the 3’ end of CTLA4 exon 4 The untranslated region contains the alleles of the (AT) n repeats. Amplification products were electrophoresed on a 12% non-denaturing polyacrylamide gel and stained with silver nitrate. Results There were 20 alleles in CTLA4 microsatellite. The frequency of the 122bp allele was significantly higher in patients with ulcerative colitis (P = 0.0001 / Pc = 0.0025, OR = 11.393, 95% CI: 2.574 to 50.429) and Crohn’s disease patients (P = 0.0003 / Pc = 0.0050, OR = 21.061, 95% CI: 3.927-112.94). Conclusion The microsatellite polymorphism of CTLA4 gene is significantly associated with IBD in Zhejiang Province.