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目的:研究三七总皂苷(Panax Notoginseng Saponins)对一次性力竭游泳运动后大鼠大脑皮质星形胶质细胞胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)表达的影响。方法:实验动物随机分为空白对照组、生理盐水组和三七总皂苷组。适应性喂养1周后,运动前30 min分别腹腔注射生理盐水和三七总皂苷,进行一次性力竭运动,分别在力竭运动后即刻和24 h灌注取材,采用免疫组织化学检测大脑皮质GFAP蛋白的表达,并记录游泳时间。结果:GFAP的阳性表达位于星形胶质细胞的细胞浆和细胞膜。生理盐水组在力竭即刻出现GFAP的表达增强,显著高于空白对照组和三七皂甙组(P<0.05),24 h后生理盐水组仍见GFAP的表达,而三七总皂苷组仅见少量GFAP的表达,二者表达未见显著差异(P>0.05);三七皂苷组与生理盐水组相比延长了大鼠的游泳时间(P<0.05)。结论:一次性力竭运动的即刻引起大鼠大脑皮质星形胶质细胞GFAP表达增强,提示与运动性中枢性疲劳的发生有关,三七总皂苷可以抑制力竭运动后星形胶质细胞GFAP的高表达,提示三七总皂苷能够延缓运动性中枢疲劳的发生,促进疲劳的消除。
Objective: To investigate the effects of Panax Notoginseng Saponins on the expression of glial fibrillary acidic protein (GFAP) in rat cerebral cortical astrocytes after a one-time exhaustive swimming exercise. Methods: Experimental animals were randomly divided into blank control group, saline group and Panax notoginseng saponins group. One week after adaptive feeding, 30 minutes before exercise, normal saline and Panax notoginseng saponins were injected intraperitoneally for one-time exhaustive exercise. The animals were perfused immediately and 24 h after exhaustive exercise, respectively. Immunohistochemistry was used to detect the changes of GFAP Protein expression, and record the swimming time. Results: The positive expression of GFAP was located in the cytoplasm and cell membrane of astrocytes. The expression of GFAP in saline group increased immediately after exhaustion, which was significantly higher than that of blank control group and notoginseng saponin group (P <0.05), and the expression of GFAP was still observed in saline group 24 h later, (P> 0.05). Compared with saline group, notoginseng saponin group prolonged the swimming time of rats (P <0.05). Conclusion: The one-time exhaustive exercise induced an immediate increase in the GFAP expression in rat cerebral cortical astrocytes, suggesting that Panax notoginseng saponins may inhibit GFAP after exhaustive exercise Of high expression, suggesting that Panax notoginseng can delay the occurrence of exercise-induced central fatigue and promote the elimination of fatigue.