论文部分内容阅读
研究了从Ａｎａｂａｎａｖａｒｉｂｉｌｉｓ中提取的４种别藻蓝蛋白ＡＰＣＩ，ＡＰＣＩＩ，ＡＰＣＩＩ及ＡＰＣＢ三聚体的稳态光谱和皮秒荧光光谱。采用Ｍｏｎｔｅ－Ｃａｒｌｏ方法对瞬态荧光光谱进行拟合，实验结果表明：ＡＰＣＩ荧光有两个带，其中第一个带位于６６２ｎｍ，有两个时间组分：３５．８ｐｓ和１．６７ｎｓ；第二个带位于６８０ｎｍ，有两个时间组分：３４．２ｐｓ和１．６４ｎｓ；ＡＰＣＩＩ瞬态荧光位于６６０ｎｍ，有两个时间组分：２０．４ｐｓ和１．６４ｎｓ；ＡＰＣＩＩ瞬态荧光位于６６０ｎｍ，有两个时间组分：２３．８ｐｓ和１．７６ｎｓ；ＡＰＣＢ瞬态荧光有两个带，其中第一个带位于６６２ｎｍ，有两个时间组分：３６．６ｐｓ和１．４５ｎｓ；第二个带位于６８０ｎｍ，有两个时间组分：２５．８ｐｓ和１．６２ｎｓ。实验结果一方面说明了藻胆体核内４种别藻蓝蛋白形成能量传递的两条途径；另一方面瞬态荧光解叠结果揭示了ＡＰＣＩ和ＡＰＣＢ三聚体内能量传递的超快过程。
The steady-state and picosecond fluorescence spectra of four allophycocyanin APCI, APCII, APCII and APCB trimer extracted from Anabanavaribilis were studied. The transient fluorescence spectra were fitted by Monte-Carlo method. The experimental results showed that there were two bands in APCI fluorescence, the first band located at 662nm with two time components: 35.8ps and 1.67ns. The second The bands are located at 680 nm with two time components: 34.2 ps and 1.64 ns. The transient fluorescence of APCII is located at 660 nm with two time components of 20.4 ps and 1.64 ns. The transient fluorescence of APCII is located at 660 nm with Two time components: 23.8ps and 1.76ns; APCB transient fluorescence with two bands, of which the first band at 662nm, there are two time components: 36.6ps and 1.45ns; the second band Located at 680 nm, there are two time components: 25.8 ps and 1.62 ns. The experimental results on the one hand shows the phycobilisome nuclear four kinds of allophycocyanin in the formation of energy transfer of the two ways; the other hand, transient fluorescence uncoupling results revealed APCI and APCB trimer energy transfer ultra-fast process.