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运用荧光光谱和紫外光谱,分别在血清蛋白的等电点以下(pH3.78)和以上(pH7.40),研究了鱼腥草的活性成分甲基正壬酮(2UC)和十二酮(2DC)与牛血清蛋白(BSA)的相互作用.结果显示在等电点前后,(2UC)和(2DC)与BSA的结合呈现可饱和模式,但对荧光的猝灭模式不同,而且结合的饱和浓度也不同.285K和pH7.40下,二者在浓度较低时呈现明显的正协同作用,而在301K时没有这种现象.计算了不同温度下结合的热力学参数△H0,△G0,△S0,结果显示疏水作用是主要的作用力.猝灭常数显示出酸度、温度和烃链长度的依赖性.考察了常见金属离子和中药活性成分对结合的影响,结果显示当共存锌离子、ANS和甘草次酸时,两种成分的加入会引起最大荧光发射波长的强烈兰移.试验表明脂肪酸2结合位(fattysite2)是它们的一个结合位点,而药物结合位点Ⅱ(siteⅡ)可能是它们的另一结合位点.
Using fluorescent and ultraviolet spectroscopy, the active ingredients of Houttuynia cordata, 2 UC and dodetone, were studied below the isoelectric point of serum protein (pH 3.78) and above (pH 7.40). 2DC) interaction with bovine serum albumin (BSA). The results showed that the binding of (2UC) and (2DC) to BSA showed a saturable pattern before and after the isoelectric point, but the fluorescence quenching mode was different, and the saturation of binding The concentrations were also different. At 285K and pH7.40, the two showed significant positive synergy at low concentrations, but not at 301K. The thermodynamic parameters of the combination of different temperatures were calculated △H0, △G0, △ S0, the results show that the hydrophobic effect is the main force. Quenching constant shows the dependence of acidity, temperature and hydrocarbon chain length. The effects of common metal ions and traditional Chinese medicine active ingredients on the binding were investigated. The results showed that when the coexisting zinc ions, ANS With glycyrrhetinic acid, the addition of the two components causes a strong blue shift in the wavelength of maximum fluorescence emission. Experiments have shown that fatty site 2 is one of their binding sites, and drug site II (site II) may be Their other binding sites.