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研究人脐静脉内皮细胞(HUVEC)和大鼠脑微血管内皮细胞(RCEC)的有丝分裂过程及其运动特征。方法用TimeLapseRecorder8050缩时录像机将人脐静脉内皮细胞及大鼠脑微血管内皮细胞活动的状态进行昼夜36h连续记录 ,然后以正常速度回放磁带 ,经MCIP图像处理系统进行图像分析 ,使细胞的有丝分裂过程在加速24倍的短时间内得到更加清晰的动态再现。结果在细胞分裂间期 ,HUVEC处于动态变化过程中 ,细胞膜以“波浪式”不停地运动 ,并不断地向四周伸出扇形伪足。而RCEC则是显现“阿米巴”样的运动并以泡状伪足向四周不断延伸。HUVEC整个有丝分裂过程所需时间为(31.5±4.4)min(n=15),而RCEC有丝分裂全过程则需(29.7±3.0)min(n=12)。结论HUVEC和RCEC有丝分裂过程所需时间没有明显区别 ,但两种细胞的运动特征却不相同 ,其机理有待深入研究
To investigate the mitotic process and the motor characteristics of human umbilical vein endothelial cells (HUVECs) and rat brain microvascular endothelial cells (RCECs). Methods TimeLapseRecorder8050 time-lapse video recorder was used to record the activity of human umbilical vein endothelial cells and rat brain microvascular endothelial cells continuously for 36 hours at day and night, and then tapes were recorded at normal speed. The images were analyzed by MCIP image processing system, Acceleration 24 times a short period of time to get a more clear dynamic reproduction. Results During the period of cell division, the HUVECs were in a dynamic process. The cell membrane kept moving in a “wave-like” mode and continuously extended fan-shaped pseudopods around. The RCEC is the manifestation of “amoeba” -like movement and bubble-like pseudopods continue to extend to the surrounding. The time required for mitosis in HUVEC was (31.5 ± 4.4) min (n = 15), while it was 29.7 ± 3.0 min (n = 12) in RCEC mitosis. Conclusion There is no significant difference in the time required for mitosis between HUVECs and RCECs, but the motility characteristics of the two kinds of cells are not the same. The mechanism needs to be further studied