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目的:研究心肌梗死患者活化蛋白C抵抗(APCR)和凝血因子Ⅴ基因3种多态性及高同型半胱氨酸血症(Hcy)的关系。方法:心肌梗死患者和正常对照血浆中APCR检测采用APTT为基础的凝固法,Hcy检测采用微循环酶法,并用限制性内切酶片段多态性方法测定FⅤG1691-A、G1091-C、A1090-G等3种基因多态性的发生情况。结果:心肌梗死患者APCR阳性占16.25%,Hcy阳性占57.12%,1例维吾尔族患者患者3种基因多态性阳性。结论:心肌梗死患者Hcy阳性率明显高于正常对照。Hcy可能是引起心肌梗死患者脑血栓形成的重要原因之一。心肌梗死患者存在APCR现象,维吾尔族人可能和凝血因子Ⅴ基因3种多态性有关。
OBJECTIVE: To study the relationship between activator protein C resistance (APCR) and coagulation factor Ⅴ gene polymorphisms and hyperhomocysteinemia (Hcy) in patients with myocardial infarction. Methods: The APTT-based coagulation method was used to detect APCR in plasma of patients with myocardial infarction and normal control. The microcirculation enzyme method was used to detect Hcy and FG1691-A, G1091-C and A1090- G and other three kinds of gene polymorphism. Results: The APCR positive rate was 16.25% in myocardial infarction patients and 57.12% in Hcy positive patients. The 3 gene polymorphisms in one Uighur patient were positive. Conclusion: The positive rate of Hcy in patients with myocardial infarction was significantly higher than that of normal controls. Hcy may be one of the important causes of cerebral thrombosis in patients with myocardial infarction. APCR phenomenon exists in patients with myocardial infarction, Uygur may be related to three polymorphisms of factor Ⅴ gene.