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采用人工催产和干法受精技术,对框鳞镜鲤♀×青鱼♂进行了2次杂交试验,共获受精卵878.9万粒,用网箱和环道孵化方法共得到培育4d仔鱼358万尾。受精率94.15%,孵化率51.4%。水温控制在23～24℃,受精后5min胚盘隆起,约58min进入2细胞期,1h10min进入4细胞期,1h25min进入8细胞期,1h43min进入16细胞期,1h53min进入32细胞期,2h20min进入多细胞期,2h49min进入囊胚早期,4h30min进入囊胚中期,5h18min进入囊胚晚期,6h30min进入原肠早期,7h30min进入原肠中期,9h30min进入原肠晚期,10h20min进入神经胚期,12h00min进入胚孔封闭期,14h30min进入眼泡出现期,18h20min进入尾芽期,23h30min进入晶体出现期,25h00min进入肌肉效应期,27h55min进入心脏搏动期,40h33min至45h30min为孵出期。胚后发育观察到4日龄,4日龄后下塘培育乌仔,该杂交组合胚胎发育时间介于双亲之间。同时,观察了杂种胚胎发育各时期中自卵裂、囊胚、原肠作用、器官形成至孵出期的发育异常现象,畸形率为51.8%。
A total of 878900 fertilized eggs were obtained from the test of carp × carp × herring ♂ using the technique of artificial ovulation and dry fertilization. In total, 3.58 million larvae of 4d larvae were obtained by cages and loop incubation methods. Fertilization rate of 94.15%, hatching rate of 51.4%. The water temperature was controlled at 23 ~ 24 ℃, 5 minutes after fertilization, the blastoderm was swollen and reached the stage of 2-cell at 58 minutes and 4-cell stage at 1h10min, 8-cell stage at 1h25min, 16-cell stage at 1h43min, 32-cell stage at 1h53min and multicellular 2h49min into the early blastocyst, 4h30min into the blastocyst metaphase, 5h18min into the blastocyst late, 6h30min into the intestine early, 7h30min into the intestine midgut, 9h30min enter the intestine late stage, 10h20min into the embryonic stage, 12h00min into the embryo closed period , 14h30min into the emergence of the eye bubble, 18h20min into the tail bud, 23h30min into the crystal appearance, 25h00min into the muscle effect, 27h55min into the heart beat period, 40h33min to 45h30min for the incubation period. Embryonic development observed 4 days old, 4 days after the next pond cultivate Aberdeen, the hybrid combination of embryonic development time between parents. At the same time, we observed the developmental abnormalities such as cleavage, blastocyst, gastrulation and organs formation to hatching in each stage of hybrid embryo development. The deformity rate was 51.8%.