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【目的】人工合成小麦具有很多优良性状,是现代小麦遗传改良的重要基因资源。本研究以具有相同AB基因组、不同D基因组的人工合成小麦材料Syn79(S79)和Syn80(S80)为供试材料,研究在氮磷钾胁迫下其生长、养分积累、养分分配和利用,差异,为小麦抗逆性基因定位和抗逆性遗传改良提供依据。【方法】采用盆栽试验,以施用N 0.20、P2O50.15和K2O 0.15 g/kg土为正常氮磷钾水平,以不施氮磷钾作为胁迫,设立4个处理:NPK(CK)、N0PK、NP0K、NPK0。小麦整个生育期每隔1个月调查1次株高和分蘖,成熟期,将小麦分根、茎叶、颖壳(穗)和子粒4个部分整理样品,收集株高、有效分蘖数、根长、穗长、根重、茎叶重、穗重、籽粒重、小穗数、穗粒数、穗粒重、千粒重和结实率。【结果】D基因组来源不同的人工合成小麦S79和S80在氮磷钾胁迫下生长、养分积累、分配和利用方面差异很大。从生长角度看,S80对氮磷胁迫敏感性低于S79,其在氮磷胁迫下长势优于S79,主要表现为株高、有效分蘖、分蘖成穗率、单株茎叶、颖壳和籽粒重等农艺性状显著好于S79。S80在氮磷钾胁迫下株高、根长和根冠比显著增加,S79的显著降低。S79在钾胁迫下农艺性状表现好于S80,主要表现为低钾环境下S79单株根重、茎叶重、颖壳重和籽粒重均高于S80。从养分积累、分配和利用看,S79在氮钾胁迫下单株氮磷钾养分积累高于S80,但S80在氮钾胁迫下的氮磷钾利用效率和收获指数均高于S79;磷胁迫下,S80单株、单株茎叶和单株颖壳中氮磷钾养分积累高于S79,但其利用效率和收获指数低于S79。S80在氮磷胁迫下籽粒中养分分配率较高,S79在钾胁迫下籽粒中养分分配较好。【结论】S79和S80在不同养分胁迫下生长、养分积累、分配和利用产生差异。S80耐低氮低磷,S79耐低钾;S80在氮钾胁迫下对氮磷钾养分利用较高,S79在磷胁迫下对氮磷钾养分利用较高。S80在养分胁迫下通过增加根长扩大养分供给范围,增加养分供给量,满足作物生长,加快养分向穗部转运,降低对籽粒产量影响。
【Objective】 Synthetic wheat has many excellent traits and is an important genetic resource for the genetic improvement of modern wheat. In this study, Syn79 (S79) and Syn80 (S80), a synthetic wheat material with the same AB genome and different D genomes, were used to study the effects of N, P and K on the growth, nutrient accumulation, This provided the basis for genetic improvement of stress resistance genes and resistance in wheat. 【Method】 Pot experiments were conducted to evaluate the effects of N, P2O5, K2O and 0.15 g / kg K2O on N, P and K fertilization. NPK (CK), N0PK, NP0K, NPK0. The whole plant growth period of wheat was surveyed once every other month for plant height, tillering and maturity. Wheat roots, stems and leaves, glume shells and seeds were sorted into 4 parts. The plant height, effective tiller number and root Length, spike length, root weight, stem and leaf weight, spike weight, grain weight, spikelet number, spikelet number, spike weight, grain weight and seed setting rate. 【Result】 The results showed that S79 and S80, different synthetic sources of D genome, had different growth, nutrient accumulation, distribution and utilization under N, P and K stress. From the perspective of growth, S80 was less sensitive to nitrogen and phosphorus stress than to S79, which grew better than S79 under nitrogen and phosphorus stress. The main manifestations were plant height, effective tillering, tillering rate, stem and leaf, glume and grain Heavy agronomic traits were significantly better than S79. Under nitrogen, phosphorus and potassium stress, plant height, root length and root / shoot ratio of S80 were significantly increased while S79 was significantly decreased. The agronomic trait of S79 was better than that of S80 under the stress of potassium stress, which mainly showed that the root weight, stem weight, glume weight and grain weight of S79 were all higher than that of S80 in low potassium environment. From accumulation, allocation and utilization of nutrients, the accumulation of N, P and K in S79 under nitrogen and potassium stress was higher than that in S80, but the N80, P use efficiency and harvest index of S80 under nitrogen and potassium stress were both higher than those under S79. Under phosphorus stress , N80, P and K accumulation in single plant, single plant stem and single glume of plant were higher than that of S79, but their utilization efficiency and harvest index were lower than that of S79. Under nitrogen and phosphorus stress, S80 had higher nutrient partitioning rate in grain, while S79 had better nutrient allocation in grain under potassium stress. 【Conclusion】 S79 and S80 have different growth, nutrient accumulation, distribution and utilization under different nutrient stress. S80 was tolerant to low-N and P-deficient and S79 was resistant to low-K. S80 had higher utilization of N, P, and K under nitrogen and potassium stress and S79 had a higher utilization of N, P and K under phosphorus stress. Under the condition of nutrient stress, S80 can increase the nutrient supply range by increasing the root length, increase the nutrient supply, meet the crop growth, accelerate the nutrient transport to the spikes and reduce the impact on the grain yield.