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目的:研究芹菜素(Apigenin,APG)对大鼠肝脏局部缺血再灌注损伤的保护作用。方法:将动物随机分为假手术组、缺血再灌注组、芹菜素(5、10、20、40、80和120mg/kg)预处理组和金纳多(4mg/kg)预处理组,术前三天开始尾静脉给药,分别检测各组大鼠肝脏组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽硫转移酶(GST)活性和丙二醛(MDA)含量,分析血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)和乳酸脱氢酶(LDH)含量的变化、并通过HE染色观察肝脏组织病理学改变和TUNEL染色观察肝脏组织细胞凋亡状况。结果:芹菜素治疗组大鼠肝脏组织中SOD、GSH-Px和GST活性显著升高,MDA含量显著降低;血清中ALT、AST和LDH水平显著降低,肝脏组织形态学改变和肝细胞凋亡状况均显著减轻;起始有效剂量为10mg/kg,有效剂量范围为10~80mg/kg,其中以20mg/kg预防效果最为显著。结论:芹菜素能够有效降低自由基损伤、改善肝功能、抑制细胞凋亡和组织病理学改变,提示芹菜素对肝脏局部缺血再灌注损伤具有保护作用。
Objective: To study the protective effect of apigenin (APG) on hepatic ischemia-reperfusion injury in rats. Methods: The animals were randomly divided into sham operation group, ischemia reperfusion group, apigenin preconditioning group (5,10,20,40,80 and 120mg / kg) and pretreatment group of Jinnuo (4mg / kg) Three days before the start of the tail vein administration, were detected in each group of liver tissue SOD, GSH-Px, GSH (GST ) Activity and malondialdehyde (MDA) content in serum were measured. The content of ALT, AST and LDH in serum were analyzed. The pathological changes of hepatic tissue and TUNEL staining were observed by HE staining. Observe the apoptosis status of liver tissue. Results: The activities of SOD, GSH-Px and GST in the liver tissue of apigenin-treated group were significantly increased, the content of MDA was significantly decreased, the levels of ALT, AST and LDH in serum were significantly decreased, the morphological changes of liver and the apoptosis of hepatocytes Were significantly reduced; the initial effective dose of 10mg / kg, the effective dose range of 10 ~ 80mg / kg, of which 20mg / kg prevention is the most significant. Conclusion: Apigenin can effectively reduce free radical injury, improve liver function, inhibit apoptosis and histopathological changes, indicating that apigenin has a protective effect on hepatic ischemia-reperfusion injury.