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目的:观察不同胎龄的新生儿在氧疗前后超氧化物歧化酶(SOD)、丙二醛(MDA)的变化,探讨它们在ROP发生中的作用。方法:选择2006年1月～2007年9月需氧疗的早产儿32例作为A组;足月儿26例作为B组。对照组为同期住院的不需氧疗的早产儿20例作为C组,足月儿18例作为D组。于吸氧前(0天)、吸氧第3天(3天)、第7天(7天)采集静脉血测定SOD的活性及MDA的含量。结果:①SOD:早产儿出生时血清SOD活性较足月儿低(P<0.01),吸氧3天与7天时,A组(分别为61.716±12.605、50.057±14.053)明显低于C组(72.265±15.422、72.346±14.784),P<0.05,且A组于3天与7天时血清SOD活性与吸氧前(72.822±10.859)相比明显降低(P<0.001);B组于吸氧3天时SOD水平(87.014±10.222)与吸氧前(91.161±10.877)相比差异无统计学意义(P>0.05),而7天时(67.755±9.730)明显低于吸氧前水平(P<0.0001),且7天时与对照组D组(90.728±13.877)相比差异有统计学意义(P<0.0001)。②MDA:A组于吸氧后3天与7天时血清MDA水平(分别为6.83±1.52、7.19±0.98)均高于吸氧前(5.62±1.38),P<0.05。吸氧7天时与对照组C组7天时(5.89±0.92)相比明显升高(P<0.01);B组吸氧后3天血清MDA水平(3.44±0.62)与吸氧前(3.06±0.28)相比差异无统计学意义(P>0.05),7天时血清MDA水平(4.47±0.81)高于吸氧前(P<0.01),且明显高于对照组D组(3.01±0.52),P<0.01。结论:早产儿较足月儿对脂质过氧化损伤更为敏感,且早产儿在高氧环境下缺乏自我保护,更加容易受到氧自由基的损伤。
OBJECTIVE: To observe the changes of superoxide dismutase (SOD) and malondialdehyde (MDA) in neonates with different gestational age before and after oxygen therapy and to explore their roles in the occurrence of ROP. Methods: 32 cases of premature infants who underwent aerobic therapy between January 2006 and September 2007 were selected as group A, and 26 cases of term infants were selected as group B. In the control group, 20 premature infants who did not need oxygen therapy were enrolled as group C in the same period and 18 full-term infants as group D. The activity of SOD and MDA content were measured before oxygen inhalation (day 0), oxygen inhalation day 3 (day 3) and day 7 (day 7). Results: ①SOD: The activity of serum SOD in preterm infants was lower than that in term infants (P <0.01), and in A group (61.716 ± 12.605 and 50.057 ± 14.053, respectively) at 3 and 7 days after oxygen inhalation ± 15.422,72.346 ± 14.784), P <0.05, and the serum SOD activity in group A at 3 days and 7 days was significantly lower than that before oxygen inhalation (72.822 ± 10.859) (P <0.001) There was no significant difference in the level of SOD between the two groups (87.014 ± 10.222) and 91.161 ± 10.877 (P> 0.05) before 7 days (67.755 ± 9.730) And there was significant difference between the 7th day and the control group D (90.728 ± 13.877) (P <0.0001). ② MDA level in group A was higher than that before oxygen inhalation (5.62 ± 1.38, P <0.05) at 3 and 7 days after oxygen inhalation (6.83 ± 1.52 and 7.19 ± 0.98, respectively). The level of serum MDA (3.44 ± 0.62) on the 3rd day after oxygen inhalation in group B was significantly higher than that of the control group (3.06 ± 0.28) on the 7th day after oxygen inhalation (5.89 ± 0.92) (P> 0.05). Serum MDA level at 4 days was higher than that before oxygen inhalation (4.47 ± 0.81) (P <0.01), and was significantly higher than that in control group (3.01 ± 0.52), P <0.01. Conclusion: Preterm infants are more sensitive to lipid peroxidation than full - term infants, and the premature infants lack self - protection in hyperoxia environment and are more likely to be injured by oxygen free radicals.