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根据核苷酸序列分析,已证明自人胎盘和下丘脑提取出的黄体生成素释放激素(LHRH)的前体在结构上是相同的。这一 LHRH 的前体被称为GnRH 相关肽(GAP)。免疫组化研究证明,在LHRH 神经元中存在 GAP 的抗原决定簇,并与LHRH 分泌颗粒共存于大鼠正中隆起的神经末梢中。在离体培养的大鼠垂体细胞,GAP 具有很强的抑制泌乳素(PRL)释放和促进促性腺激素释放的作用。以上结果提示,促性腺激素和 PRL 的中枢调控可能是通过在下丘脑神经元中合成某种共用的 LHRH-PRL 释放抑制因子(PIF)的前体偶联在一起。为进一步阐明 GAP 的 PRL 释放抑制活性,本文观察 GAP 对基础条件下以及由乙醚应激和吸吮(suckle)引起的 PRL 释放的影响。
Based on nucleotide sequence analysis, precursors of luteinizing hormone-releasing hormone (LHRH) extracted from human placenta and hypothalamus have been shown to be structurally identical. This precursor of LHRH is known as GnRH-related peptide (GAP). Immunohistochemical studies have demonstrated that there are antigenic determinants of GAP in LHRH neurons and coexist with LHRH secreting particles in the rat’s median dorsal nerve endings. In vitro cultured rat pituitary cells, GAP has a strong inhibition of prolactin (PRL) release and promote gonadotropin release. The above results suggest that central regulation of gonadotrophin and PRL may be coupled by the synthesis of a common precursor of LHRH-PRL release-inhibiting factor (PIF) in hypothalamic neurons. To further elucidate the PRL release inhibitory activity of GAP, we examined the effect of GAP on PRL release under basal conditions and by ether stress and suckle.