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目的探讨参芎注射液对腹膜纤维化大鼠腹膜组织中结缔组织生长因子(connective tissue growth factor,CTGF)表达的影响。方法用4.25%腹膜透析液+脂多糖建立大鼠腹膜纤维化模型。实验分正常对照组(A)、模型组(B)、低剂量参芎注射液治疗组(C)、高剂量参芎注射液治疗组(D)。实时定量聚合酶链反应(real-time polymerase chain reaction,RT-PCR)检测CTGF mRNA的表达,间接免疫荧光及免疫印迹法检测蛋白的表达水平。结果与A组相比,B、C、D组的CTGF mRNA和蛋白的表达水平明显上调;与B组相比,C、D明显减轻(P<0.05)。结论参芎注射液可能通过抑制腹膜纤维化大鼠腹膜组织的CTGF表达而发挥一定的抗纤维化作用。
Objective To investigate the effect of Shenxiong injection on the expression of connective tissue growth factor (CTGF) in peritoneal tissue of rats with peritoneal fibrosis. Methods 4.25% peritoneal dialysate + lipopolysaccharide was used to establish rat peritoneal fibrosis model. The experiment was divided into normal control group (A), model group (B), low dose Shenxiong injection treatment group (C) and high dose Shenxiong injection treatment group (D). The expression of CTGF mRNA was detected by real-time polymerase chain reaction (RT-PCR), and the expression of CTGF mRNA was detected by indirect immunofluorescence and Western blotting. Results Compared with group A, the expression of CTGF mRNA and protein in group B, C and D were significantly increased. Compared with group B, C and D were significantly reduced (P <0.05). Conclusion Shenxiong injection may exert anti-fibrosis effect by inhibiting CTGF expression in peritoneal tissue of peritoneal fibrosis rats.