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目的 研究胰岛素样生长因子 Ⅰ (IGF Ⅰ )基因表达异常及其与糖尿病外周病变的关系。方法 用四氧嘧啶诱发糖尿病大鼠模型。逆转录 聚合酶链反应半定量分析坐骨神经IGF ⅠmRNA含量 ;酶联免疫吸附法分析组织IGF Ⅰ肽含量 ;诱发肌电图测定坐骨神经电生理指标 ;光镜、电镜下观察坐骨神经形态学改变。结果 病程早期 ,非严格控制糖尿病组大鼠坐骨神经电生理指标与正常非糖尿病对照组差异显著 ,神经组织IGF ⅠmRNA含量 (0 430± 0 0 31vs 0 370± 0 0 16、0 2 80±0 0 10 ,P值 <0 0 1,<0 0 0 1)、肽含量 (IGF Ⅰng/mg总蛋白 )均下降 (38 44± 3 6 0vs 30 0 6± 3 6 0、2 3 71± 2 70 ,P值 <0 0 1,<0 0 0 1) ,程度均随糖尿病控制状态而异 ,且随病程进展逐渐下降 (IGF ⅠmRNA :0 32 0± 0 0 2 1～ 0 2 30± 0 0 6 0 ;IGF Ⅰ肽 :2 8 80± 3 30～ 19 5 1± 1 80 ) ,与坐骨神经功能 (感觉神经传导速度 :r =0 714,P <0 0 0 1;诱发电位波幅 :r =0 716 ,P <0 0 0 1)、组织形态异常 (轴突面积 :r =0 81,P <0 0 0 1)关系密切 ,血糖正常糖尿病组大鼠与正常对照组间上述指标差异无显著性。结论 糖尿病早期即出现组织IGF Ⅰ基因表达下降 ,程度依糖尿病严重状态而异并随疾病进程加重 ,这种表达异常
Objective To investigate the relationship between abnormal gene expression of insulin-like growth factor Ⅰ (IGF Ⅰ) and peripheral blood of diabetic patients. Methods Alloxan induced diabetic rat model. Quantitative analysis of IGF Ⅰ mRNA in sciatic nerve by reverse transcription polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), electrophysiological index of sciatic nerve induced by electromyography, morphological changes of sciatic nerve under light microscope and electron microscope. Results Early in the course of the disease, the sciatic nerve electrophysiological indexes in non-strictly controlled diabetic rats were significantly different from those in normal non-diabetic control rats. The levels of IGF Ⅰ mRNA in nerve tissues (0 430 ± 0 0 31 vs 0 370 ± 0 0 16, 0 80 ± 0 0 10 (P <0 01, <0 0 0 1), and the content of peptide (IGF Ⅰ ng / mg total protein) decreased (38 44 ± 36 0 vs 30 0 6 ± 3600, 23 71 ± 2 70, P Values <0 0 1, <0 0 0 1), the degree of which varies with the state of diabetes control, and gradually decreases with the progress of the disease (IGF Ⅰ mRNA: 0 32 0 ± 0 0 2 1 ~ 0 2 30 ± 0 0 6 0; IGF Ⅰ peptide: 2880 ± 3 30 ~ 19 5 1 ± 1 80), and sciatic nerve function (sensory nerve conduction velocity: r = 0 714, P <0 0 01; evoked potential amplitude: r = 0 716, P There was no significant difference in histological abnormalities (axon area: r = 0 81, P <0.01). There was no significant difference in the above indexes between the normal blood glucose group and the normal control group. Conclusion The expression of IGF Ⅰ gene in the early stage of diabetes decreased, which was different with the severity of diabetes mellitus and aggravated with the progression of the disease. This abnormal expression