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目的 :探讨BALB/C和C5 7BL/6小鼠创伤反应差异性的分子遗传学机制。方法 :利用基因芯片技术对BALB/C和C5 7BL/6小鼠在创伤后 1h和 6h肝组织基因表达谱进行比较研究 ,对在两种小鼠创伤反应早期差异表达的基因进行聚类分析。结果 :对 132 7条差异表达的基因进行聚类分析后发现有两大族基因的表达在BALB/C小鼠和C5 7BL/6小鼠创伤后早期存在明显差异。BALB/C小鼠糖类代谢、脂类代谢和激素代谢活跃 ,细胞信号转导以G蛋白信号通路和Ca2 + 信号通路激活为主 ,而且与组织损伤相关的基因也出现高表达。而C5 7BL/6小鼠急性时相反应和免疫反应被激活 ,细胞凋亡途径被抑制 ,细胞信号转导以酪氨酸蛋白激酶系统激活为主要特征。结论 :BALB/C和C5 7BL/6小鼠的创伤反应差异性可能与它们在创伤后基因组的表达方式不同相关。
Objective: To investigate the molecular genetic mechanism of the difference of trauma response between BALB / C and C5 7BL / 6 mice. Methods: The gene expression profiles of liver tissues in BALB / C and C5 7BL / 6 mice at 1 h and 6 h after trauma were compared by gene chip technology. The genes differentially expressed in the early stage of trauma reaction were clustered. Results: Cluster analysis of 132 7 differentially expressed genes showed that there were significant differences in the expression of two genera in early stage after BALB / C mice and C5 7BL / 6 mice. The carbohydrate metabolism, lipid metabolism and hormone metabolism of BALB / C mice were active. The signal transduction of GST and Ca2 + signal transduction pathway in BALB / C mice was mainly activated, and the expression of genes related to tissue damage was also observed. However, the acute phase reaction and immune response were activated in C5 7BL / 6 mice, and the apoptotic pathway was inhibited. The activation of cell signal transduction was the main feature of tyrosine kinase system. CONCLUSIONS: The differences in the traumatic response between BALB / C and C5 7BL / 6 mice may be related to their different ways of expressing the genome after trauma.