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目的探讨不同饮食结构对茶碱在大鼠体内的药动学影响。方法 36只大鼠分为对照组(空腹给药)、标准饮食组(给药前给予标准饮食)和高脂饮食组(给药前给予高脂饮食),各组大鼠灌胃给予氨茶碱,采用高效液相色谱法测定各个时间点血中的茶碱浓度,并用DAS2.0软件计算药动学参数。结果对照组、标准饮食组和高脂饮食组的主要动力学参数如下:血药浓度时间-曲线面积为(305.239±68.728)、(239.927±51.837)和(245.264±53.630)μg·h-1·ml-1,平均驻留时间为(6.935±0.904)、(7.239±0.936)和(7.144±0.661)h,半衰期为(5.066±1.098)、(5.480±1.441)和(5.119±1.230)h,达峰时间为(1.167±0.389)、(1.833±0.389)和(1.917±0.289)h,清除率为(0.069±0.017)、(0.086±0.018)和(0.085±0.018)L·h·kg,峰浓度为-1-1(52.485±10.472)、(35.901±7.534)和(33.309±6.255)μg·ml-1。标准饮食和高脂饮食均可使峰浓度减少,高脂饮食可使达峰时间延长。结论饮食可使茶碱在大鼠体内的药动学参数发生改变。
Objective To investigate the effects of different dietary structures on the pharmacokinetics of theophylline in rats. Methods 36 rats were divided into control group (fasting), standard diet group (standard diet before administration) and high fat diet group (high fat diet before administration) The concentrations of theophylline in blood at various time points were determined by HPLC. The pharmacokinetic parameters were calculated by DAS2.0 software. Results The main kinetic parameters of control group, standard diet group and high-fat diet group were as follows: the time-curve area of plasma concentration was (305.239 ± 68.728), (239.927 ± 51.837) and (245.264 ± 53.630) μg · h-1 · The average residence time was (6.935 ± 0.904), (7.239 ± 0.936) and (7.144 ± 0.661) h, and the half-lives were 5.066 ± 1.098, 5.480 ± 1.441 and 5.119 ± 1.230 h, respectively The peak time was (1.167 ± 0.389), (1.833 ± 0.389) and (1.917 ± 0.289) h, and the clearance rates were (0.069 ± 0.017), 0.086 ± 0.018 and 0.085 ± 0.018 L · h · kg, Was -1-1 (52.485 ± 10.472), (35.901 ± 7.534) and (33.309 ± 6.255) μg · ml-1, respectively. The standard diet and high-fat diet can reduce the peak concentration, high-fat diet can peak time can be extended. Conclusion Diet can change the pharmacokinetic parameters of theophylline in rats.