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血红蛋白的珠蛋白的肽链常用分析法有:(1)羧甲基纤维素柱层析法;(2)巯基乙醇—尿素解离后的电泳分析法;(3)对氯汞苯甲酸解离后的电泳分析法。但有的操作繁琐、费时;有的分辨力差,不能同时分析各种肽链,而且均不能分离HbF的γ链的两种变异体:G_γ和A_γ。Rovera等结合巯
Commonly used analytical methods of hemoglobin globin chain peptide: (1) carboxymethyl cellulose column chromatography; (2) mercaptoethanol - electrophoresis after dissociation of urea; (3) Chloromer mercuric benzoate dissociation After the electrophoresis analysis. However, some cumbersome and time-consuming operations; some poor resolution, can not simultaneously analyze a variety of peptide chains, and can not separate the HbF γ chain of two variants: G_γ and A_γ. Rovera et al