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目的:探索2种激光显微切割(laser microdissection)系统应用于切割分离小鼠牙胚特定组织的不同特点。方法:以莱卡LMD6000和蔡司PALM激光显微切割系统分别获取CD1小鼠胚胎期17 d和出生后2 d的下颌第一磨牙牙囊和牙乳头组织。以Trizol法提取2套切割系统获取组织中总RNA,再以RT-PCR法检测两者中管家基因GAPDH表达量的差异。结果:应用莱卡LMD系统获得小鼠下颌第一磨牙牙胚组织中GAPDH基因表达量显著高于以蔡司的PALM系统获得组织中相同基因表达量。莱卡LMD6000激光显微切割系统操作界面友好,切割组织迅速。结论:莱卡LMD6000更适合于下游芯片实验对提取组织RNA完整度较高的要求。
Objective: To explore the different characteristics of two laser microdissection systems applied to the isolation and isolation of specific tissues of mouse tooth germs. Methods: The dentin and dental papilla tissues of the mandibular first molar were obtained on the 17th day after embryogenesis and 2 days after birth in Lycra LMD6000 and Zeiss PALM laser microdissection system respectively. Trizol method was used to extract two sets of cleavage system to obtain the total RNA in the tissue. The difference of the expression of housekeeping gene GAPDH between the two groups was detected by RT-PCR. Results: The expression level of GAPDH gene in tooth germ of mouse mandibular first molar obtained by Lycra LMD system was significantly higher than that of the same gene obtained from tissue with Zeiss PALM system. Lycra LMD6000 laser micro-cutting system user-friendly interface, cutting tissue quickly. Conclusion: Lycra LMD6000 is more suitable for the downstream chip experiments to extract the higher integrity of tissue RNA requirements.