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目的探讨男性不育者精液中一氧化氮(NO)和顶体酶(ACE)、透明质酸酶(HYD)、酸性磷酸酶(ACP)的变化。方法参照世界卫生组织(WHO)标准方法进行精液常规分析,按精子密度、活动率不同分组。采用镀铜镉还原荧光法检测NO代谢产物硝酸盐(NO3-);用改良的Kennedy法检测精子ACE活性;用Singer法测定HYD活性;采用磷酸苯二钠法检测ACP。结果72例不育各组NO含量明显高于正常生育组(P<0.01),而ACE、HYD、ACP活性则明显低于正常生育组(P<0.01),随精子密度、活动率减少和畸形率增高而NO含量升高,ACE、HYD、ACP活性降低。结论精液酶类是评价精子成熟、活动率及穿卵受精能力的重要指标,NO对精子酶活性有抑制灭活作用,可影响精子的受精能力,对男性不育的诊断治疗具有重要价值。
Objective To investigate the changes of nitric oxide (NO), acrosin (ACE), hyaluronidase (HYD) and acid phosphatase (ACP) in male infertility. Methods According to the World Health Organization (WHO) standard methods for routine analysis of semen, according to the sperm density, activity rates were divided into groups. The nitric oxide (NO3-) was detected by copper-cadmium reduction fluorescence method. The sperm ACE activity was detected by modified Kennedy method. The HYD activity was measured by Singer method. Results The content of NO in 72 infertile groups was significantly higher than that in normal fertility group (P <0.01), while the activities of ACE, HYD and ACP were significantly lower than those in normal fertility group (P <0.01) Increasing the rate of NO content increased, ACE, HYD, ACP activity decreased. Conclusions Semen enzymes are important indexes for evaluating sperm maturation, activity rate and ability to penetrate eggs. NO inhibits the inactivation of sperm enzyme activity and may affect spermatozoa fertility, which is of great value to the diagnosis and treatment of male infertility.