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目的 观察动脉粥样硬化家兔红细胞L 精氨酸 (L Arg)转运及一氧化氮合酶 (NOS)活性的变化 ,探讨动脉粥样硬化发病机制。方法 选家兔 12只 ,分为两组 ,每组 6只 ,分别喂以高脂饮食(高脂组 )及普通饮食 (对照组 ) 6周 ,取静脉血浆测血脂水平。并测定红细胞的L Arg转运及NOS活性。结果 (1)高脂组动物血浆总胆固醇 (Total Chol)、甘油三脂 (TG)及低密度脂蛋白胆固醇 (LDL C)水平明显高于对照组 (P <0 0 1) ;(2 )高脂组动物红细胞L Arg最大转运速率 (Vmax)较对照组下降19 1% (P <0 0 1) ,NOS活性较对照组降低 42 4% (P <0 0 1)。结论 动脉粥样硬化时红细胞L 精氨酸的跨膜转运障碍及一氧化氮合酶活性降低。
Objective To investigate the changes of L Arg transport and nitric oxide synthase (NOS) activity in atherosclerotic rabbit erythrocytes and to explore the pathogenesis of atherosclerosis. Methods Twelve rabbits were randomly divided into two groups with 6 rats in each group, which were fed with high-fat diet (high-fat diet) and normal diet (control group) for 6 weeks, respectively. The L Arg transport and NOS activity of erythrocytes were measured. Results (1) Total cholesterol, triglyceride (TG) and low density lipoprotein cholesterol (LDL C) levels in high fat diet group were significantly higher than those in control group (P <0.01); (2) Compared with the control group, the maximal transport rate of L Arg in lipid group decreased 19.1% (P <0.01) and the NOS activity decreased 42 42% (P <0.01) in the control group. Conclusion Atherosclerosis erythrocyte L-arginine transmembrane dysfunction and nitric oxide synthase activity decreased.