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幽门螺旋菌(HP)与慢性胃炎及十二指肠溃疡密切相关,其在胃癌发生中的作用是目前人们所关注的。本研究旨在调查胃癌高危人群新奥尔良黑人中产细胞毒素HP菌株的感染情况。材料和方法:86例(黑人为主,均属于社会经济下层)全部是HP感染者,包括十二指肠溃疡13例、胃溃疡22例和非溃疡性消化不良51例。在胃窦和胃体部取活检做组织学检查和HP培养,同时取其血清作HP的ELISA和细胞毒素中和试验。86例共培养得144HP菌株。将细菌培养上清液加于HeLa S3细胞系孵育24～48小时,显微镜下观察以20％细胞出现胞浆
Helicobacter pylori (HP) is closely related to chronic gastritis and duodenal ulcer, and its role in the development of gastric cancer is currently of concern. The aim of this study was to investigate the infection status of mid-toxin-producing cells in New Orleans, a genus of cytotoxic HP from patients at high risk for gastric cancer. MATERIALS AND METHODS: Totally 86 patients (mainly blacks, all belonging to the socioeconomic) were all HP-infected, including 13 cases of duodenal ulcer, 22 cases of gastric ulcer and 51 cases of non-ulcer dyspepsia. Histological examination and HP culture were taken from the gastric antrum and the corpus. The serum was also used for ELISA and cytotoxicity neutralization test. 86 cases co-cultured 144HP strains. The bacterial culture supernatant was added to HeLa S3 cell line incubated for 24 to 48 hours under a microscope to 20% of the cells appear cytoplasm