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目的:探讨不同碳氮源对胶质芽孢杆菌GSY-1的生物脱硅效果的影响。方法:采用单因素试验并结合方差分析确定影响GSY-1生物脱硅的碳氮源种类及浓度,通过回归实验及响应面分析进一步优化培养条件,然后利用10L发酵罐进行放大实验验证。结果:研究发现,乳糖和尿素对GSY-1生物脱硅的影响均显著,其中尿素10g/L及乳糖13g/L时,培养液中铝硅比(A/S)分别可由2.84提高到5.45和5.42,通过响应面优化实验,确定尿素10.35g/L、乳糖12.60g/L时,菌株GSY-1的脱硅效果最佳,铝硅比由2.84提高到5.67,增幅达99.65%。经10 L发酵罐放大实验,测得浸矿后的铝硅比为5.07,较浸矿前提高78.52%。结论:乳糖和尿素对胶质芽孢杆菌GSY-1脱硅效果影响显著,响应面法可有效用于GSY-1菌株脱硅条件的优化。
Objective: To investigate the effect of different carbon and nitrogen sources on the bio-desiliconization of Bacillus gluconate GSY-1. Methods: The types and concentrations of carbon and nitrogen sources affecting the biological desiliconization of GSY-1 were determined by single factor test and analysis of variance. The culture conditions were further optimized by regression analysis and response surface analysis, and then the amplification was verified by 10L fermenter. Results: The results showed that the effects of lactose and urea on the desilication of GSY-1 were all significant. When the urea concentration was 10g / L and lactose was 13g / L, the A / S ratio in the culture medium increased from 2.84 to 5.45 and 5.42. Through the response surface optimization experiment, it was found that GS11 was the best one with 10.35 g / L urea and 12.60 g / L lactose, and the ratio of Al to Si increased from 2.84 to 5.67 with an increase of 99.65%. After 10 L fermenter amplification experiment, measured after leaching ore Al-Si ratio of 5.07, 78.52% higher than before the leaching. Conclusion: The effects of lactose and urea on the desilication of Bacillus gluconate GSY-1 are significant. Response surface methodology can be used to optimize the desalination conditions of GSY-1.