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【目的】观察滋肾育胎丸对900 MHz拟手机辐射雌性大鼠卵巢功能和卵巢ATM蛋白表达的影响。【方法】选用30只SD雌性大鼠随机分为正常组、辐射组、滋肾育胎丸组,正常组不接受辐射,辐射组和滋肾育胎丸组每天暴露于自制900 MHz手机辐射源4 h,连续30 d,滋肾育胎丸组每天灌胃滋肾育胎丸悬液,另外2组灌胃等容积生理盐水,阴道涂片选动情期处死大鼠。采用放射免疫法检测各组大鼠血清睾酮(T)、雌二醇(E2)含量,苏木精—伊红(HE)染色观察卵巢组织形态学变化,免疫组织化学法检测大鼠卵巢ATM蛋白表达。【结果】与正常组比较,辐射组大鼠血清E2水平显著降低(P<0.05),血清T含量无显著变化(P>0.05);辐射组卵巢颗粒细胞层数减少(P<0.05),颗粒细胞核ATM蛋白表达减弱;与辐射组大鼠比较,滋肾育胎丸组大鼠血清E2水平显著升高,卵巢颗粒细胞层数增多,ATM蛋白表达显著增强(均P<0.05)。【结论】拟手机辐射对大鼠卵巢功能有不良影响,可能与影响卵巢ATM蛋白表达有关;滋肾育胎丸对手机辐射大鼠卵巢损伤具有保护作用。
【Objective】 To observe the effects of Zishen Yutai Wan on the ovarian function and the ovarian ATM protein expression in a 900 MHz radiosensitized female rat. 【Methods】 Thirty SD female rats were randomly divided into normal group, radiation group and Zishenyutong pill group. Normal group did not receive radiation. Radiation group and Zishenyutong pill group were exposed daily to a homemade 900 MHz cell phone radiation source 4 h for 30 days. The rats in Zishen Yutongwan group were fed with Zishen Yutai pill suspension every day. The other two groups were given gavage with equal volume of normal saline and vaginal smear. The levels of serum testosterone (T) and estradiol (E2) were detected by radioimmunoassay. The morphological changes of ovarian tissue were observed by hematoxylin-eosin (HE) staining. The expression of ATM protein expression. 【Results】 Compared with the normal group, the level of serum E2 was significantly decreased (P <0.05) and the level of serum T was not changed (P> 0.05). The number of ovarian granulosa cells in the irradiation group was decreased (P <0.05) The expression of ATM protein in the nucleus was weakened. Compared with the radiation group, the level of serum E2, the number of granulosa cells and the expression of ATM protein in Zishenyutai Pills group were significantly increased (all P <0.05). 【Conclusion】 The cell phone radiation may have an adverse effect on ovarian function in rats, which may be related to the expression of ATM protein in ovaries. Zishen Yutai Pill has a protective effect on ovary injury induced by cell phone radiation in rats.