论文部分内容阅读
目的 :研究不同浓度的Fas对胃印戒细胞癌细胞株的影响。方法 :采用细胞染色法观察细胞凋亡的形态 ,MTT法检测细胞增殖情况 ,原位末端标记法 (TUNEL法 )检测凋亡细胞 ,流式细胞仪检测细胞的DNA含量及凋亡百分率。结果 :2～ 8μg/ml的Fas均可抑制胃印戒细胞癌细胞增殖且诱导细胞发生凋亡。结论 :Fas有明显抑制胃印戒细胞癌细胞株生长及促进细胞凋亡的作用
Objective: To study the effect of different concentrations of Fas on gastric signet-ring cell line. METHODS: Cellular staining was used to observe the morphology of cell apoptosis. MTT assay was used to detect cell proliferation. Apoptotic cells were detected by TUNEL method. Flow cytometry was used to detect DNA content and percentage of apoptosis. RESULTS: Fas of 2-8 μg/ml inhibited the proliferation of gastric signet ring cells and induced apoptosis. Conclusion: Fas significantly inhibits the growth of gastric signet-ring cell line and promotes apoptosis.