作者对新生大鼠睾丸组织冷冻保存方法及保存后的睾丸组织移植进行了研究。结果表明，用二甲基亚砜作低温保护剂，４℃渗透平衡３０～４５ｍｉｎ以０．４℃／ｍｉｎ的速率降温贮存于－８０℃低温冰箱，冻存１个月和冻存３个月的睾丸组织快速复温后在体外组织培养条件下，具有良好的合成和释放睾酮的能力。将冻存１个月和３个月的睾丸组织移植到去势成龄雄性大鼠体内，移植物具有生长发育和分泌睾酮的机能。该实验结果为人类胎儿睾丸组织的冷冻保存及组织移植提供了实验基础。文章尚对二甲基亚砜对睾丸组织的损伤效应作了讨论。 The authors studied the cryopreservation of newborn rat testis tissue and testicular tissue transplantation after preservation. The results showed that with DMSO as cryoprotectant, the cells were stored at -80 ℃ in a low-temperature refrigerator at a rate of 0.4 ℃ / min for 30-45 min at 4 ℃, frozen for 1 month and frozen for 3 months Of testicular tissue after rapid rewarming in vitro tissue culture conditions, has a good ability to synthesize and release testosterone. Testicular tissues frozen for 1 month and 3 months were transplanted into castrated adult male rats. The implants had the function of growth and secretion of testosterone. The experimental results provide experimental basis for cryopreservation and tissue transplantation of human fetus testis tissue. The article also on dimethyl sulfoxide damage to testicular tissue were discussed.