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研究了对虾中T-2毒素急性暴露的残留规律,并探明其对雄性小鼠的遗传毒性效应。实验以LC-MS/MS检测肌肉注射染毒后,对虾中T-2毒素残留量,并经灌胃,检测毒虾中T-2毒素对小鼠遗传毒性的危害。结果发现,对虾中游离态T-2毒素的残留量与暴露剂量呈正相关,表现蓄积性,并且不同组织的蓄积量差异较大,以肌肉中含量最低,接近于零。经毒虾灌胃后,小鼠的精子畸形率及微核率也随暴露剂量逐渐增大。与对照组相比,高剂量灌胃组的小鼠精子畸形率和微核率出现显著性变化(P<0.05),高剂量组的四个对虾组织都具有毒性,其中以对虾肌肉和肝胰腺的毒性最强,其次为肠道。表明T-2毒素在对虾体内具有隐蔽性,特别是在肌肉中,游离态T-2毒素易于被转化为隐蔽态T-2毒素,从逃避检测,并产生二次危害隐患。
Residues of acute exposure of T-2 toxin in shrimp were studied and their genotoxic effects on male mice were investigated. T-2 toxin residues in shrimp were detected by LC-MS / MS after intramuscular injection, and the toxicity of T-2 toxin in mice was tested by gavage. The results showed that the residual T-2 toxin in shrimp was positively correlated with the exposure dose, showing accumulation, and the accumulation volume of different tissues was quite different, with the content of muscle in the lowest, close to zero. Shrimp poisoned shrimp, mouse sperm deformity rate and micronucleus rate also gradually increased with the exposure dose. Compared with the control group, the sperm deformity rate and micronucleus rate of mice in the high-dose gavage group showed significant changes (P <0.05), and the four shrimp tissues in the high-dose group were toxic. Among them, shrimp muscle and hepatopancreas The most toxic, followed by the intestinal tract. T-2 toxin shows that the toxin is hidden in shrimp, especially in the muscle, free T-2 toxin can easily be converted into a concealed T-2 toxin to evade detection and cause secondary hazard.