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目的:优选决明子蛋白质的最佳提取工艺及体外消化率测定。方法:采用正交试验法,以凯氏定氮法测得决明子蛋白质的含量和得率为考察指标,对提取过程中缓冲液浓度、pH值、固液比及提取时间4个工艺因素进行优化研究,并用胃蛋白酶-胰蛋白酶法测定体外消化率。结果:决明子蛋白质最佳提取工艺为KH2PO4-NaOH缓冲液浓度为50 mmol/L,pH值为8.0,液固液比为1∶50,提取12h。影响决明子蛋白提取各因素的主次关系为:缓冲液浓度>pH值>固液比>提取时间,其中缓冲液浓度和pH值的影响达显著性水平。优化参数下提取的决明子粗蛋白含量为93.0%,提取率为94.2%,体外消化率为97.6%。结论:决明子是含蛋白质较高的植物种子,其蛋白质消化率较高,具有很好的开发前景。
Objective: To optimize the extraction process of cassia protein and the determination of in vitro digestibility. Methods: The content and yield of cassia seed protein determined by Kjeldahl method were investigated by orthogonal test. The factors including buffer concentration, pH value, solid-liquid ratio and extraction time were optimized In vitro digestibility was determined by pepsin-trypsin assay. Results: The optimum extraction process of cassia seed protein was as follows: the concentration of KH2PO4-NaOH buffer was 50 mmol / L, the pH was 8.0, the ratio of liquid to solid was 1:50 and the extraction time was 12h. The main relationship between the factors affecting the extraction of cassia seed protein: buffer concentration> pH> solid-liquid ratio> extraction time, in which buffer concentration and pH value of the impact reached a significant level. The crude protein content of cassia seed extracted under the optimized parameters was 93.0%, the extraction rate was 94.2% and the in vitro digestibility was 97.6%. Conclusion: Cassia seed is a plant protein with high protein content, which has high protein digestibility and has good development prospect.