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目的探讨IL-10在子痫前期氧化应激发病机制中的作用。方法采集经剖宫产子痫前期患者及同期正常妊娠孕妇胎盘组织各15例为研究对象。用实时定量PCR检测胎盘组织IL-10、NOX1 mRNA的表达。比色法检测胎盘组织MDA含量。统计比较数据并相关分析。结果与正常妊娠组相比较,子痫前期患者胎盘IL-10 mRNA表达水平降低(P<0.05)。NOX1 mRNA表达水平升高(P<0.05)。差异有统计学意义。同时MDA含量明显升高(P<0.01)。相关分析显示子痫前期患者胎盘组织IL-10 mRNA表达水平与MDA含量呈负相关(P<0.05)。与NOX1 mRNA表达水平呈负相关(P<0.01)。结论子痫前期患者胎盘组织氧化应激水平升高,与IL-10密切相关。IL-10可能是通过降低NOX1的表达来减轻胎盘氧化应激。
Objective To investigate the role of IL-10 in the pathogenesis of oxidative stress in preeclampsia. Methods Fifteen cases of cesarean section in preeclampsia and normal pregnant women of the same period were enrolled in this study. Real-time quantitative PCR was used to detect the expression of IL-10 and NOX1 mRNA in placenta. Colorimetric assay of placental MDA content. Statistics and comparative analysis of data. Results Compared with normal pregnancy group, placental IL-10 mRNA expression was decreased in preeclampsia patients (P <0.05). NOX1 mRNA expression increased (P <0.05). The difference was statistically significant. At the same time MDA content was significantly increased (P <0.01). Correlation analysis showed that there was a negative correlation between the level of IL-10 mRNA and the content of MDA in placenta of preeclampsia (P <0.05). And NOX1 mRNA expression was negatively correlated (P <0.01). Conclusion The level of oxidative stress in placenta of preeclampsia patients is elevated, which is closely related to IL-10. IL-10 may reduce placental oxidative stress by decreasing NOX1 expression.